AI Article Synopsis
- Understanding how cells control access to DNA information is crucial in the postgenome era, particularly through the role of chromatin, which is formed by DNA wrapped around histone proteins.
- Histone modifications after translation are key in regulating how DNA-dependent proteins interact with the DNA, thus affecting gene expression and chromatin structure.
- Chromatin immunoprecipitation (ChIP) is a technique that allows scientists to study the status of histone modifications and nuclear proteins at specific genes, providing insights into chromatin dynamics in response to various stimuli.
Article Abstract
In this postgenome era, understanding how a cell regulates access to information encoded in the deoxyribonucleic acid (DNA) is essential. In eukaryotic cells, DNA is bound to histone proteins to form chromatin fibers. Numerous studies have now shown that post-translational histone modifications play an important role in regulating the access of DNA-dependent proteins to the DNA template. Determining the status of histone modifications in a genomic region has proven to yield information on the chromatin structure and the regulation of a specific gene in vivo. Chromatin immunoprecipitation (ChIP) allows researchers to determine the status of both histone modifications and the nuclear effector proteins located at gene of interest. ChIP, if applied globally, can also reveal how chromatin structures are dynamically changed when cells respond to certain stimuli. In this chapter, we describe this powerful technique in detail.
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| http://dx.doi.org/10.1385/1-59745-005-7:261 | DOI Listing |
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