AI Article Synopsis
- A new method using gas chromatography-mass spectrometry (GC-MS) analyzes ergosterol in single kernels and ground barley/wheat, involving saponification and a simple hexane extraction process.
- Recovery rates for ergosterol from ground barley ranged from 88.5% to 97.1%, while single kernel recoveries were between 93.0% and 95.9%.
- This efficient method allows one person to process 80 samples a day, making it useful for screening cereal cultivars for fungal resistance and studying early fungal invasions.
Article Abstract
A method for analyzing ergosterol in a single kernel and ground barley and wheat was developed using gas chromatography-mass spectrometry (GC-MS). Samples were saponified in methanolic KOH. Ergosterol was extracted by "one step" hexane extraction and subsequently silylated by N-trimethylsilylimidazole/trimethylchlorosilane (TMSI/TMCS) reagent at room temperature. The recoveries of ergosterol from ground barley were 96.6, 97.1, 97.1, 88.5, and 90.3% at the levels of 0.2, 1, 5, 10, and 20 microg/g (ppm), respectively. The recoveries from a single kernel were between 93.0 and 95.9%. The precision (coefficient of variance) of the method was in the range 0.8-12.3%. The method detection limit (MDL) and the method quantification limit (MQL) were 18.5 and 55.6 ng/g (ppb), respectively. The ergosterol analysis method developed can be used to handle 80 samples daily by one person, making it suitable for screening cereal cultivars for resistance to fungal infection. The ability for detecting low levels of ergosterol in a single kernel provides a tool to investigate early fungal invasion and to study mechanisms of resistance to fungal diseases.
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| http://dx.doi.org/10.1021/jf060149f | DOI Listing |
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