Specific identification of Bacillus anthracis (B. anthracis) is vital for the accurate treatment of afflicted personnel during biological warfare situations and civilian terrorist attacks. In order to accomplish this, we have subjected the lysates from B. anthracis to affinity purification using monoclonal antibodies for the selected antigenic protein present in the bacteria. The bound antigenic protein was identified by multi-dimensional protein identification technology (MudPIT) to be a surface layer protein EA1. The same antigen was identified from the lysates from a few strains of B. anthracis demonstrating the observation to be common for B. anthracis strains. Hence, this presents an effective pathway for the identification of the bacteria present in unknown samples of various origins. Generation of a database containing the EA1 protein has been found to be useful in the database search of unknown samples.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/rcm.2564 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Probable Case of Cutaneous Anthrax with Toxic Manifestations and Fatality seen in an adolescent in Sokoto, Nigeria: A postmortem review.
Niger Med J
January 2025
Department of Medical Microbiology, Usman Danfodiyo University Teaching Hospital, Sokoto, Nigeria.
Background: Anthrax is a life-threatening zoonotic disease caused by Gram-positive, spore-forming bacterium . It manifests as a cutaneous, gastrointestinal, and respiratory disease. The cutaneous form ranges from a self-limiting lesion to severe edematous lesions with toxemic shock.
View Article and Find Full Text PDFOptimized PCF architectures for THz detection of aquatic pathogens: Enhancing water quality monitoring.
PLoS One
January 2025
Department of Electrical and Electronic Engineering, Pabna University of Science and Technology, Pabna, Bangladesh.
Waterborne bacteria pose a serious hazard to human health, hence a precise detection method is required to identify them. A photonic crystal fiber sensor that takes into account the dangers of aquatic bacteria has been suggested, and its optical characteristics in the THz range have been quantitatively assessed. The PCF sensor was designed and examined as computed in Comsol Multiphysics, a program in which uses the method of "Finite Element Method" (FEM).
View Article and Find Full Text PDFCutaneous Anthrax: What is the Hallmark?
Acta Med Indones
October 2024
Division of Tropical and Infection Diseases, Department of Internal Medicine, Medical Faculty of Universitas Sebelas Maret - Moewardi Hospital, Surakarta, Indonesia.
A 71-year-old man complained of a blackish wound under his left eye, which began with fever and reddish spots after helping to slaughter a cow and cut its meat. The fever occured especially in the afternoon to evening, and is not accompanied by chills and sweating. On day 4 of fever onset, the fever diminished and the spots progressively widened with swelling.
View Article and Find Full Text PDFThe anthrax pathogen can remain dormant as spores in soil for many years. This applies to both natural foci and to sites of anthropogenic activity such as tanneries, abattoirs, or wool factories. The A.
View Article and Find Full Text PDFIdentification of Strains from Animal Cases in Ethiopia and Genetic Characterization by Whole-Genome Sequencing.
Pathogens
January 2025
Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa P.O. Box 1176, Ethiopia.
Anthrax is a zoonotic disease characterized by rapid onset with usual fatal outcomes in livestock and wildlife. In Ethiopia, anthrax is a persistent disease; however, there are limited data on the isolation and molecular characterization of strains. This study aimed to characterize isolated from animal anthrax outbreaks between 2019 and 2024, from different localities in Ethiopia.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!