The indispensability of biotin for crucial processes like lipid biosynthesis coupled to the absence of the biotin biosynthesis pathway in humans make the enzymes of this pathway, attractive targets for development of novel drugs against numerous pathogens including M. tuberculosis. We report the spectral and kinetic characterization of the Mycobacterium tuberculosis 7,8-Diaminopelargonic acid (DAPA) synthase, the second enzyme of the biotin biosynthesis pathway. In contrast to the E. coli enzyme, no quinonoid intermediate was detected during the steady state reaction between the enzyme and S-adenosyl-L-methionine (SAM). The second order rate constant for this half of the reaction was determined to be 1.75 +/- 0.11 M-1s-1. The Km values for 7-keto-8-aminopelargonic acid (KAPA) and SAM are 2.83 microM and 308.28 microM, respectively whereas the Vmax and kcat values for the enzyme are 0.02074 micromoles/min/ml and 0.003 s-1, respectively. Our initial studies pave the way for further detailed mechanistic and kinetic characterization of the enzyme.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/15216540600746997 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Statistical optimization of pectinases from thermophilic Aspergillus fumigatus BT-4 employing response surface methodology through submerged fermentation using agricultural wastes.
BMC Biotechnol
January 2025
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, China.
Background: In this study, thermophilic pectinase-producing strains were isolated. Among all the isolates, strain No. 4 was identified as Aspergillus fumigatus BT-4 based on its morphology and 18 S rDNA analysis.
View Article and Find Full Text PDFOptimization of hypobaric and ultrasonic processing of persimmon rhamnogalacturonan-I to enhance drug-digestion interactions.
Int J Biol Macromol
January 2025
Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche (IDiBE), Universidad Miguel Hernández, 03202 Elche, Alicante, Spain. Electronic address:
The biological activity of polysaccharides used for nutraceuticals/drug excipients has been a neglected area of study. This work deals with the preparation, optimization, characterization, and evaluation of persimmon (Diospyros kaki Thunb.) fruit by-products and the study of the resultant dietary fiber (DF) interaction with other compounds, using acetaminophen as a model.
View Article and Find Full Text PDFSubstrate-specific responses to mixing conditions in high-solids enzymatic hydrolysis: Insights from microcrystalline cellulose and dilute-acid pretreated corncob.
Int J Biol Macromol
January 2025
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest, Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, PR China. Electronic address:
This study investigates the mixing effects on the enzymatic hydrolysis of microcrystalline cellulose (MCC) and dilute-acid pretreated corncob substrates under high-solid conditions. Enzymatic hydrolysis experiments were conducted to assess cellulose conversion rates under varying mixing conditions (0, 50, 150, and 250 rpm) and solids loadings (5 %, 15 %, 25 %, and 35 %, w/v), and distinct physicochemical properties of the substrates were characterized. Additionally, the role of mixing conditions and solid loadings on cellulose hydrolysis kinetics and enzyme adsorption on both substrates and lignin were elucidated.
View Article and Find Full Text PDFComparative photocatalytic degradation of cationic rhodamine B and anionic bromocresol green using reduced ZnO: A detailed kinetic modeling approach.
Chemosphere
January 2025
Center for Green Chemistry and Environmental Biotechnology, Ghent University Global Campus, 119-5 Songdomunhwa-Ro, Yeonsu-Gu, Incheon, 406-840 South Korea; Department of Green Chemistry and Technology, Faculty of Bioscience Engineering, Ghent University, 653 Coupure Links, Ghent, B-9000, Belgium. Electronic address:
The photocatalytic degradation of rhodamine B (RhB), a cationic dye, and bromocresol green (BCG), an anionic dye, was investigated using oxygen vacancy-enriched ZnO as the catalyst. These dyes were selected due to their differing charges and molecular structures, allowing for a deeper exploration of how these characteristics impact the degradation process. The catalyst was prepared by reducing ZnO with 10% H/Ar gas at 500°C, and the introduction of oxygen vacancies was confirmed using various characterization techniques.
View Article and Find Full Text PDFMagnetic nanoparticle modified moss Biochar: A novel solution for effective removal of enrofloxacin from aquaculture water.
J Environ Manage
January 2025
Wuxi Fisheries College, Nanjing Agricultural University, 214081, Wuxi, PR China; Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, 214081 Wuxi, PR China; Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Environmental Factors (Wuxi), Ministry of Agriculture and Rural Affairs, 214081, Wuxi, PR China; Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture and Rural Affairs, 100000, Beijing, PR China; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, 214081, Wuxi, PR China. Electronic address:
The presence of residual antibiotics in water constitutes a potential threat to aquatic environments. Therefore, designing environmentally friendly and efficient biochar adsorbents is crucial. Aquaculture by-product moss (bryophyte) was transformed into biochar, which can eliminate antibiotics from wastewater through adsorption.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!