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http://dx.doi.org/10.1042/bj0800193 | DOI Listing |
J Environ Manage
December 2024
Tshwane University of Technology, Department of Chemistry, Private Bag X680, Pretoria, 0001, South Africa.
Landfilling is common in developing countries since it is the easiest and cheapest way of waste disposal, however, it leads to serious environmental problems such as soil, water, and air pollution. A landfill has a life span of fifteen years after which it is closed leaving the site unusable, as a result, effective methods are needed for restoring and reclaiming the closed landfill site for future use. Phytoremediation has emerged as a viable and environmentally friendly method, which uses green plants to remove pollutants from soil, air, and water.
View Article and Find Full Text PDFJ Sep Sci
January 2025
Department of Biosciences and Medical Biology, University of Salzburg, Salzburg, Austria.
Imaged capillary isoelectric focusing was successfully applied for separating an in-house synthesized closely related peptide pair, that is, a linear 12-mer (Rp5-L) and its cyclic 15-mer variant (Rp5-C). Rp5-L represents a mimotope, that is, an epitope mimicking peptide, of the CD20 antigen, which is over-expressed in B-cell-related tumors. Peptide identity-including the successful disulfide bond formation in Rp5-C-was confirmed with matrix-assisted laser desorption ionization-time of flight mass spectrometry.
View Article and Find Full Text PDFJ Sep Sci
January 2025
Department of Analytical, Bioanalytical Sciences and Miniaturization (LSABM) Chemistry, Biology and Innovation (CBI), UMR CNRS-ESPCI Paris 8231, ESPCI Paris, PSL University, CNRS, Paris, France.
Adduction on protein nucleophile sites by mustard agents can be monitored to assess detection of retrospective exposure to these agents. Cysteine 34 (Cys34) on human serum albumin was selected as the target of choice. This work targets di- and tripeptides adducted on Cys34 by sulfur mustard, sesquimustard, and nitrogen mustards separated in hydrophilic liquid chromatography (HILIC) and Reversed-Phase (RP) mode.
View Article and Find Full Text PDFBiomed Chromatogr
January 2025
Beijing Harmony Health Medical Diagnostics Co., Ltd., Beijing, China.
In the context of personalized and precision medicine, simultaneous monitoring of different forms of vitamins A and E and their metabolites could help us better understand the status of vitamins A and E in the body. The aim of this study was to establish a method for simultaneous determination of 13 kinds of vitamins A and E and their metabolites in human serum. Serum samples were directly detected by LC-MS/MS after deproteinization.
View Article and Find Full Text PDFJ Chromatogr A
December 2024
Freie Universität Berlin, Institut für Chemie und Biochemie, Thielallee 63, 14195 Berlin, Germany.
Analytical thin layer chromatography (TLC) is a simple yet powerful chromatographic technique that is widely used for the qualitative characterization of complex mixtures such as plant extracts. For their qualitative and visual characterisation, a large number of more or less specific colour reactions are at hand and numerous reference substances are available as well. However, the identification of extract components by colour and the comparison of retention times is not straightforward.
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