AI Article Synopsis
- The study investigates if methylation at GATC sites in the dnaA promoter influences the timing of initiation events at oriC.
- Point mutations were introduced to remove three (TGW1) and five (TGW2) of the six GATC sites in the dnaA promoter.
- Despite these mutations, all strains still initiated chromosomal replication in a synchronized manner.
Article Abstract
To examine whether methylation of the GATC sites present in the dnaA promoter region is responsible for the strict temporal coordination of initiation events at oriC as measured by the synchrony of initiation, we introduced point mutations eliminating three (TGW1) and five (TGW2) of the six GATC sites present in the dnaA promoter region. All of the strains containing these mutations, including the one with five GATC sites eliminated, initiated chromosomal replication synchronously.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1482936 | PMC |
| http://dx.doi.org/10.1128/JB.00089-06 | DOI Listing |
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