A marine agarolytic bacterium, designated strain TMA1(T), was isolated from a seawater sample collected in a shallow-water region of An-Ping Harbour, Taiwan. It was non-fermentative and Gram-negative. Cells grown in broth cultures were straight or curved rods, non-motile and non-flagellated. The isolate required NaCl for growth and exhibited optimal growth at 25 degrees C and 3 % NaCl. It grew aerobically and was incapable of anaerobic growth by fermenting glucose or other carbohydrates. Predominant cellular fatty acids were C(16 : 0) (17.5 %), C(17 : 1)omega8c (12.8 %), C(17 : 0) (11.1 %), C(15 : 0) iso 2-OH/C(16 : 1)omega7c (8.6 %) and C(13 : 0) (7.3 %). The DNA G + C content was 41.0 mol%. Phylogenetic, phenotypic and chemotaxonomic data accumulated in this study revealed that the isolate could be classified in a novel species of the genus Thalassomonas in the family Colwelliaceae. The name Thalassomonas agarivorans sp. nov. is proposed for the novel species, with TMA1(T) (=BCRC 17492(T) = JCM 13379(T)) as the type strain.
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http://dx.doi.org/10.1099/ijs.0.64130-0 | DOI Listing |
Appl Microbiol Biotechnol
October 2019
Department of Biological Sciences, Myongji University, Yongin, Gyeonggido, 449-728, Republic of Korea.
Although many β-agarases that hydrolyze the β-1,4 linkages of agarose have been biochemically characterized, only three α-agarases that hydrolyze the α-1,3 linkages are reported to date. In this study, a new α-agarase, AgaWS5, from Catenovulum sediminis WS1-A, a new agar-degrading marine bacterium, was biochemically characterized. AgaWS5 belongs to the glycoside hydrolase (GH) 96 family.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
April 2014
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
A Gram-staining-negative, aerobic, rod-shaped bacterium, designated strain T202(T), was isolated from the gill of a cultured flounder (Paralichthys olivaceus). Based on 16S rRNA gene sequence similarity, strain T202(T) was a member of the family Colwelliaceae and shared 93.32-96.
View Article and Find Full Text PDFJ Appl Microbiol
March 2014
Food Industry Research and Development Institute, Bioresource Collection and Research Center, HsinChu, Taiwan.
Aims: The agarase from Thalassomonas agarivorans BCRC 17492 was cloned and overexpressed in Escherichia coli. The characterization of the novel agarase was performed.
Methods And Results: The genomic library of T.
Antonie Van Leeuwenhoek
January 2014
University of Science and Technology (UST), 113 Gwahangno, Yuseong, Taejeon, South Korea.
A Gram-negative, aerobic, non-motile, dark brown-coloured and rod-shaped bacterial strain, designated G-MB1(T), was isolated from a tidal flat sediment of the South Sea, South Korea. Strain G-MB1(T) was found to grow optimally at 25 °C, at pH 7.0-8.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
November 2011
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
A Gram-staining-negative, motile, agarolytic bacterium, designated M-M1(T), was isolated from marine sand obtained from Geoje Island, South Sea, Korea, and its taxonomic position was investigated using a polyphasic taxonomic approach. Strain M-M1(T) grew optimally at pH 7.0-8.
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