AI Article Synopsis
- Double-stranded RNA (dsRNA) gene interference is a targeted technique used to silence specific genes, demonstrated with the allatostatin (AS)-A gene in crickets and moths.
- Injecting dsRNA into larvae and adult specimens resulted in significant and lasting decreases in mRNA levels, effective for up to a week, alongside elevated juvenile hormone levels in their blood.
- The experiment led to reduced body weight, incomplete development during molting, and decreased reproductive capabilities, including lower egg production and impaired reproductive organ development.
Article Abstract
Double-stranded RNA (dsRNA) gene interference is an efficient method to silence gene expression in a sequence specific manner. Here we show, that dsRNA targeting the allatostatin (AS)-A type (FGL/I/V-amide) gene of Gryllus bimaculatus (Ensifera, Gryllidae) and Spodoptera frugiperda (Lepidoptera, Noctuidae) injected into freshly moulted larvae or adult crickets and moths produced a rapid and long-lasting reduction in the mRNA levels in various tissues. The effect lasted up to 7 days. Following dsRNA injection, the juvenile hormone (JH) titers in the hemolymph were clearly raised in both species. AS-dsRNA injection induced a reduced body weight in larval and adult crickets and the imaginal moult was incomplete. Silencing allatostatin type-A expression also reduced the egg and testes development in crickets, and the oviposition rate was drastically diminished in both species.
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| http://dx.doi.org/10.1016/j.ibmb.2006.03.008 | DOI Listing |
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