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Determination of viable mammalian cells by luminol chemiluminescence using microperoxidase.
Anal Biochem
March 2009
Shizuoka Institute of Science and Technology, Fukuroi, Shizuoka, Japan.
Chemiluminescent assay for menadione-catalyzed H(2)O(2) production by mammalian cells was modified by luminol chemiluminescence with microperoxidase instead of peroxyoxalate chemiluminescence with carcinogenic fluorescent materials. Luminol can be used as a common chemiluminescent reagent for the determination of viable mammalian cells and bacteria.
View Article and Find Full Text PDFMenadione-catalyzed luminol luminescent assay as a novel evaluation method of ethanol tolerance in yeast cells.
Anal Biochem
February 2009
Shizuoka Institute of Science and Technology, Fukuroi, Shizuoka 437-8555, Japan.
In this study, ethanol inhibited the growth and glucose-induced proton release of yeast cells in a dose-dependent manner. On the other hand, ethanol tolerance of menadione-catalyzed luminol luminescence by yeast cells increased with increasing ethanol concentrations in the growth medium. The intracellular reduced-form nicotinamide adenine dinucleotide (NADH) concentration also increased with increasing ethanol concentrations in the medium and was enough to maintain constant menadione-catalyzed luminol luminescence.
View Article and Find Full Text PDF[Menadione-catalyzed luminol chemiluminescence assay for the rapid detection of viable bacteria].
Shokuhin Eiseigaku Zasshi
April 2006
National Food Research Institute, Ibaraki, Japan.
Menadione-catalyzed luminol chemiluminescence assay for the rapid detection of viable bacteria in foods under aerobic conditions.
J Food Prot
December 2004
National Food Research Institute, Food Hygiene Research Team, Tsukuba-shi, Ibaraki 305-8642, Japan.
A menadione-catalyzed luminol chemiluminescence assay was developed for the rapid detection and estimation of viable bacteria in foods. The principle of this assay is based on the extracellular menadione-catalyzed active oxygen spieces (O2- and H2O2) generated by the activity of NAD(P)H:menadione oxidoreductase in viable cells. This luminol chemiluminescence assay requires 10 min for the incubation of cells with menadione and then 2 s for the measurement of chemiluminescence intensity after an injection of luminol solution without the treatment of cell lysis.
View Article and Find Full Text PDFMenadione-catalyzed luminol chemiluminescent assay for viability of Mycobacterium bovis.
Microbiol Immunol
March 2003
Nikken Biomedical Laboratory, Kuze-gun, Kyoto, Japan.
Stable luminol chemiluminescence was observed 10 min after the addition of menadione to a suspension of Mycobacterium bovis homogenized in Middlebrook 7H9 broth base including OADC enrichment. The chemiluminescence intensity was proportional to the absorbance of the bacterial suspension at 600 nm in a range of 0.005 to 0.
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