We describe a filtration-based culture method to enhance sensitivity of routine culture for recovery of fungi from respiratory specimens. The new method resulted in an 8.3% (P = 0.0039) increase in recovery for all organisms and a 6.4% (P = 0.0391) for Candida species when compared to the conventional culture technique.
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http://dx.doi.org/10.1016/j.diagmicrobio.2006.04.005 | DOI Listing |
Biotechnol Bioeng
September 2024
Biologics Process Research and Development, Merck & Co., Inc., Kenilworth, New Jersey, USA.
Advances in upstream production of biologics-particularly intensified fed-batch processes beyond 10% cell solids-have severely strained harvest operations, especially depth filtration. Bioreactors containing high amounts of cell debris (more than 40% particles <10 µm in diameter) are increasingly common and drive the need for more robust depth filtration processes, while accelerated timelines emphasize the need for predictive tools to accelerate development. Both needs are constrained by the current limited mechanistic understanding of the harvest filter-feedstream system.
View Article and Find Full Text PDFMethods Mol Biol
May 2023
Department of Microbiology and Immunology, The University of North Carolina, Chapel Hill, NC, USA.
Extracellular vesicle (EV) isolation from conditioned cell culture medium has been a challenging topic. It is particularly difficult to obtain pure and intact EVs at a large scale. The commonly used methods such as differential centrifugation, ultracentrifugation, size exclusion chromatography, polyethylene glycol (PEG) precipitation, filtration, and affinity-based purification each have their advantages and limitations.
View Article and Find Full Text PDFAnn Work Expo Health
January 2023
Institute for Risk Assessment Sciences, Utrecht University, Utrecht, The Netherlands.
PLoS One
July 2022
Laboratory of Pharmaceutical Biotechnology, Ghent University, Ghent, Belgium.
In a cell-based non-invasive prenatal test (cbNIPT), intact circulating trophoblasts (CTs) are isolated from maternal blood for subsequent genetic analysis. Enrichment of these CTs from maternal blood is the most challenging step in the cbNIPT workflow. This study aims to assess the suitability of the filtration-based Metacell® technology to enrich CTs from maternal blood at week 10 to 13 of gestation.
View Article and Find Full Text PDFTalanta
May 2022
Department of Foods and Nutrition, Kookmin University, Seoul, 136-702, Republic of Korea. Electronic address:
Escherichia coli O157:H7 is a major cause of fresh vegetable-associated infections that can threaten human health. A method for rapidly detecting food-borne pathogens should be developed for safe food management. A clustered regularly interspaced short palindromic repeats (CRISPR)-based detection method has the potential to greatly advance biosensing technology through its high sensitivity and specificity.
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