AI Article Synopsis
- The study investigates how puerarin affects the growth of vascular smooth muscle cells (VSMC) stimulated by thrombin.
- It uses flow cytometry to analyze cell proliferation and Western blotting to assess changes in the proteins c-fos and bcl-2 after treatment.
- Results show that puerarin significantly reduces VSMC proliferation and DNA synthesis by inhibiting the expression of c-fos and bcl-2 proteins.
Article Abstract
Objective: To observe the role of puerarin on the proliferation of vascular smooth muscle cells(VSMC) induced by thrombin (T) and the effect of puerarin on the c-fos and bcl-2 protein expression.
Method: Cell number and cell cycle analysis using flow cytometry were adopted as two different indicators of effects on proliferation of VSMC. Western blot was used to indicate the changes of c-fos and bcl-2 protein after 24 h of treatment of T and puerarin.
Result: 1.5 x 10(-5) - 1.5 x 10(-3) mol x L(-1) puerarin could significantly suppress this stimulation of VSMC proliferation and DNA synthesis induced by T. Western blot demonstrated that after 24 hour of treatment with T and puerarin, T could significantly increase c-fos and bcl-2 protein and 1.5 x 10(-5) - 1.5 x 10(-3) mol x L(-1) puerain could significantly suppress this increase.
Conclusion: puerarin can suppress the proliferation and DNA synthesis of VSMC promoted by T. This inhibitory effects of puerarin are closely related with the suppression of c-fos and bcl-2 protein.
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