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Direct On-Chip Diagnostics of / Complex in Bovine Mastitis Using Bioinformatics-Driven Portable qPCR.
Biomolecules
December 2024
Institute of Life Sciences & Resources, Department of Food Science and Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea.
This study introduces an innovative on-site diagnostic method for rapidly detecting the / complex (SBSEC), crucial for livestock health and food safety. Through a comprehensive genomic analysis of 206 genomes, this study identified genetic markers that improved classification and addressed misclassifications, particularly in genomes labeled and . These markers were integrated into a portable quantitative polymerase chain reaction (qPCR) that can detect SBSEC species with high sensitivity (down to 10 or 10 colony-forming units/mL).
View Article and Find Full Text PDFProsthetic Joint Infection by Streptococcus lutetiensis (Bovis) With Noncancerous Polyps and Negative Blood Cultures: A Case Report.
Cureus
November 2024
Internal Medicine, Marshall University Joan C. Edwards School of Medicine, Huntington, USA.
Prosthetic joint infection (PJI), caused by Streptococcus bovis group (SBG), is uncommon and related to colorectal cancer. We present here a case of an 84-year-old male who had a past medical history of chronic obstructive pulmonary disease (COPD), congestive heart failure, pulmonary arterial hypertension, iron deficiency anemia, chronic kidney disease, diabetes mellitus, gout, hypertension, bilateral knee replacement with left knee pain and swelling. We initially suspected gout and treated him with prednisolone, but it did not relieve him.
View Article and Find Full Text PDFThe Significance of Clinical Suspicion: From Pitting Edema to Streptococcus infantarius Endocarditis.
Cureus
October 2024
Internal Medicine, University of California, Los Angeles (UCLA) - Kern Medical, Bakersfield, USA.
Article Synopsis
- * A case is presented of a patient who arrived at the emergency department with only worsening pitting edema and subtle physical examination findings, ultimately leading to the discovery of severe heart conditions linked to infective endocarditis.
- * The case highlights the significance of thorough physical examinations and clinical judgment, emphasizing the role of tubular adenomas in causing bacteremia and the necessity for a more comprehensive approach in patient assessment.
Impact of ovalbumin allergy on oral and gut microbiome dynamics in 6-week-old BALB/c mice.
Front Microbiol
September 2024
Department of Prosthodontics, The Affiliated Hospital of Qingdao University, Qingdao, China.
Article Synopsis
- - The study investigates how gut and oral microbiota influence food allergy development, specifically using ovalbumin-sensitive mice as a model to analyze these microbiota changes.
- - Results showed significant imbalances (dysbiosis) in the microbiota of ovalbumin-sensitized mice, with notable decreases in some species and increases in others, while also identifying accurate biomarkers for predicting food allergies.
- - The findings suggest that changes in microbial composition may play a crucial role in food allergy development and propose specific microbial strains as potential biomarkers for screening food allergies.
Usefulness of newer testing modalities for the accurate diagnosis of culture-negative endocarditis.
BMJ Case Rep
September 2024
Cardiovascular Disease, Internal Medicine, University of Michigan Michigan Medicine, Ann Arbor, Michigan, USA.
Article Synopsis
- A woman in her 80s with multiple health issues, including heart failure and colon cancer, was admitted for severe shortness of breath.
- Imaging showed infection on her prosthetic heart valve and pacemaker lead, but blood cultures were negative.
- A novel cell-free DNA Karius test identified a pathogen linked to her colon cancer, leading to the diagnosis of recurrent malignancy which was confirmed through further tests and subsequent surgery.
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