AI Article Synopsis
- The study focuses on isolating and characterizing a cystatin from the Chinese sturgeon, revealing it as a homolog to mammalian cystatins and providing insights into its structure.
- The cystatin cDNA is 336 nucleotides long, encoding a 112 amino acid protein, and was successfully subcloned into a yeast expression vector for production.
- The recombinant cystatin yielded approximately 215 mg/l with significant protease inhibition activity against papain and cathepsin B, and its heat stability was also evaluated.
Article Abstract
Cystatin, a superfamily of cysteine proteinase inhibitor of cathepsins and other cysteine proteinases, is widely distributed in animal tissues and body fluids. Although considerable attention has been given to mammalian and avian cystatins, little is known about cystatins from other vertebrates. In this study, a cDNA coding for Chinese sturgeon (Acipenser sinensis) cystatin was isolated and characterized. The corresponding mature cystatin peptide cDNA is 336 nucleotides long and encodes a protein of 112 amino acids. Sequence comparison showed that the cloned cystatin was a homolog of the mammalian Family II cystatin. The cystatin cDNA of Chinese sturgeon was subcloned into yeast expression vector pPICZalphaA and transformed into Pichia pastoris GS115 strain. After methanol induction, SDS-PAGE analysis of the culture supernatant indicated that the yield of recombinant cystatin was about 215 mg/l medium supernatant in shaking-flask fermentation medium, accounting for 73.6% of the total supernatant secreted proteins. Our data also showed that the recombinant cystatin is active in inhibiting the protease activity of papain and cathepsin B. Heat stability of the recombinant cystatin was also measured.
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| http://dx.doi.org/10.1016/j.jbiotec.2006.02.023 | DOI Listing |
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