AI Article Synopsis
- The study highlights that the phiC31 integrase from Streptomyces phage can facilitate the integration of foreign genes at specific sites in the genomes of humans, mice, rats, and Drosophila.
- Researchers discovered that this integrase also promotes homologous recombination between attB and pseudo attP sites in bovine cells, showing at least double the integration efficiency in bovine fibroblasts and MDBK cells.
- Two key pseudo attP sites, BpsF1 and BpsM1, were identified in the bovine genome, suggesting that the phiC31 integrase system could enhance genetic engineering efforts in cattle, benefiting ruminant research.
Article Abstract
Streptomyces phage phiC31 integrase was found to mediate site-specific integration of foreign genes at pseudo attP sites of genomes in human, mouse, rat, and Drosophila. This paper reports that phiC31 integrase can also mediate homologous recombination between attB and pseudo attP sites in bovine cells and foreign gene integration was increased at least 2-fold in bovine fibroblasts or Madin-Darby bovine kidney (MDBK) cells. Two intrinsic pseudo attP sites named BpsF1 and BpsM1 located in the inter-gene regions on chromosome 28 and 19, respectively, were identified in bovine genome. These pseudo attP sites shared similar characteristics with those from other species as previously described. Our study demonstrated that the phiC31 integrase system provides a new potential for genetic engineering of the bovine genome and might be beneficial for the research on ruminant.
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| http://dx.doi.org/10.1016/j.bbrc.2006.04.145 | DOI Listing |
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