We assess the influence of geometric aberrations on the in-plane performance of optical traps by studying the dynamics of trapped colloidal spheres in deliberately distorted holographic optical tweezers. The lateral stiffness of the traps turns out to be insensitive to moderate amounts of coma, astigmatism, and spherical aberration. Moreover holographic aberration correction enables us to compensate inherent shortcomings in the optical train, thereby adaptively improving its performance. We also demonstrate the effects of geometric aberrations on the intensity profiles of optical vortices, whose readily measured deformations suggest a method for rapidly estimating and correcting geometric aberrations in holographic trapping systems.
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Discov Nano
January 2025
Institute of Medical Research, Northwestern Polytechnical University, 127 West Youyi Road, Xi'an, 710072, Shaanxi, China.
Human lens epithelial cells (hLECs) are critical for lens transparency, and their aberrant metabolic activity and gene expression can lead to cataract. Intracellular delivery to hLECs, especially to sub-cellular organelles (e.g.
View Article and Find Full Text PDFNat Struct Mol Biol
January 2025
Laboratory of Structural Biophysics and Mechanobiology, The Rockefeller University, New York, NY, USA.
Fascin cross-links actin filaments (F-actin) into bundles that support tubular membrane protrusions including filopodia and stereocilia. Fascin dysregulation drives aberrant cell migration during metastasis, and fascin inhibitors are under development as cancer therapeutics. Here, we use cryo-EM, cryo-electron tomography coupled with custom denoising and computational modeling to probe human fascin-1's F-actin cross-linking mechanisms across spatial scales.
View Article and Find Full Text PDFTalanta
December 2024
Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350108, China. Electronic address:
The flap endonuclease 1 (FEN1) plays a key role in DNA replication and repair, its aberrant expression is associated with tumor development, so it has been recognized as a promising biomarker for a variety of cancers. Here, a novel "turn on" mode gold nanocube-enhanced surface-enhanced Raman scattering (SERS) biosensor was constructed by combining a heated Au electrode (HAuE), exonuclease III (Exo III)-assisted cycle amplification, and gold nanocube (AuNC)-based SERS enhancement to achieve highly sensitive detection of FEN1 activity. The SERS tag was prepared using the Raman reporter modified on the AuNC surface, and the high electromagnetic field provided by the sharp geometric feature of AuNC greatly enhanced the SERS signal.
View Article and Find Full Text PDFRev Sci Instrum
December 2024
Institute of Planetary Research, German Aerospace Center (DLR), Rutherfordstr. 2, 12489 Berlin, Germany.
Comet 67P/Churyumov-Gerasimenko (hereafter 67P) was the primary target of ESA's Rosetta mission. Hyperspectral images acquired by the Mapping channel of the Visible and InfraRed Thermal Imaging Spectrometer aboard Rosetta can be used to derive physical and compositional surface properties by detailed spectrophotometric analyses. This calls for a precise spatial co-registration between measurements and geometry information.
View Article and Find Full Text PDFSci Rep
November 2024
MOE Key Laboratory of TianQin Mission, TianQin Research Center for Gravitational Physics and School of Physics and Astronomy, Frontiers Science Center for TianQin, Gravitational Wave Research Center of CNSA, Sun Yat-sen University (Zhuhai Campus), Zhuhai, 519082, China.
As a critical payload of the gravitational wave detection interferometry system, the tilt-to-length (TTL) noise has a significant influence on the detection accuracy of the interferometry system. The non-geometric TTL (NG-TTL) noise, which is the main component of the TTL noise within the telescope, is closely related to the sensitive aberrations of the system's small pupil. Due to the different environments of the earth and space, structural deformations of the telescope can cause significantly unfavorable changes in the sensitive aberrations at the small pupil.
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