AI Article Synopsis
- The study outlines the creation and testing of molecules that combine arginine-rich transporters with luciferin, enabling luciferin to be released only after these molecules enter specific cells with luciferase.
- Each time luciferin is released inside the cell, it emits photons that can be detected, providing a way to measure how much luciferin is taken up and released in real-time.
- This method allows researchers to analyze the effects of different transporter types and linker variations on luciferin uptake and release.
Article Abstract
The design, synthesis, and evaluation of conjugates of arginine-rich transporters and luciferin are described that release luciferin only after entry into cells that are stably transfected with luciferase. Each molecule of free luciferin that is released after entry generates a photon that can be measured allowing for real-time quantification of uptake and release in cells. The process provides a method to assay uptake and release of free luciferin as a function of variations in the releasable linker and in the transporter.
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| http://dx.doi.org/10.1021/ja0586283 | DOI Listing |
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