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[Screening and identification of metastasis-related gene expression in tongue carcinoma with cDNA microarray assay]. | LitMetric

AI Article Synopsis

  • The objective of the study was to find genes related to metastasis in tongue carcinoma by comparing mRNA expression profiles from two cell strains with different metastatic potentials using microarray technology.
  • The research utilized two cell lines, Tca8113 and Tb, employing fluorescent cDNA probes for hybridization to a chip with 1,152 human genes; gene expression was then analyzed and validated through RT-PCR.
  • The findings revealed 37 genes with significantly different expression levels between the two cell lines, indicating possible roles in tumor immunity, cell adhesion, and growth control, providing insights for future early diagnosis and treatment options.

Article Abstract

Objective: To identify metastasis-associated genes in tongue carcinoma and to better understand the mechanism underlying tongue carcinoma metastasis. To compared mRNA expression profiles of two tongue carcinoma cell strains with high and low metastatic potentials using microarray technology.

Methods: Tca8113 and Tb cells were used as model systems to study the molecular mechanism of tumor metastasis. Two fluorescent cDNA probes labeled with Cy3 and Cy5 dyes were prepared from the mRNA samples of Tca8113 and Tb cells by reverse transcription method. The two color probes were then mixed and hybridized to the cDNA chip constructed by double dots of 1 152 human genes, and scanned at two wave lengths. Differential expression genes from the above two cell lines were analyzed using computer. Then six of the different expression genes were further validated by RT-PCR technique.

Results: In the 1 152 clones of known genes and expressed sequence tags that were analyzed, 37 showed significantly different (minimum 2 folds) expression levels in two cell lines. Among the 37 genes, 15 were up regulated (with ratio more than 2) and 22 down regulated (with ratio less than 1/2). The results of RT-PCR analysis were coincident with those of microarray assay.

Conclusion: Some of these genes are known to be involved in human tumor antigen, immune surveillance, adhesion, cell signaling pathway and growth control. It is suggested that the microarray in combination with a relevant analysis facilitates rapid and simultaneous identification of multiple genes of interests and in this study it provided a profound clue to screen candidate targets for early diagnosis and intervention.

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