In the subventricular zone (SVZ) of the adult mammalian brain, neural stem cells continually produce transit-amplifying precursors, which generate neuroblasts migrating into the olfactory bulb. Previous studies have suggested that SVZ cells also have the capacity to generate some striatal neurons after cerebral ischemia. The infusion of epidermal growth factor (EGF) has been demonstrated to increase the number of these regenerated neurons. However, which cell types in the SVZ are stimulated to proliferate or differentiate after EGF infusion remains unknown. In this paper, we demonstrated that cerebral ischemia results in an increase in the number of EGF receptor (EGFR)-positive transit-amplifying cells in the SVZ. EGF infusion into the ischemic brain caused the number of transit-amplifying cells to increase and the number of neuroblasts to decrease. On the other hand, after an interval of 6 days after the discontinuation of EGF infusion, a significant increase in the number of neuroblasts was found, both in the striatum and the SVZ. These results suggest that the replacement of neurons in injured striatum can be enhanced by an EGF-induced expansion of transit-amplifying cells in the SVZ.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.neulet.2006.04.039 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Fate mapping in mouse demonstrates early secretory differentiation directly from Lgr5+ intestinal stem cells.
Dev Cell
January 2025
Novo Nordisk Foundation Center for Stem Cell Medicine, reNEW, Faculty of Health and Medical Sciences, University of Copenhagen, DK-2200 Copenhagen N, Denmark. Electronic address:
The intestinal epithelium has a remarkably high turnover in homeostasis. It remains unresolved how this is orchestrated at the cellular level and how the behavior of stem and progenitor cells ensures tissue maintenance. To address this, we combined quantitative fate mapping in three complementary mouse models with mathematical modeling and single-cell RNA sequencing.
View Article and Find Full Text PDFDirect and indirect RANK and CD40 signaling regulate the maintenance of thymic epithelial cell frequency and properties in the adult thymus.
Front Immunol
December 2024
Laboratory for Immune Homeostasis, RIKEN Center of Integrative Medical Sciences, Yokohama, Japan.
Medullary thymic epithelial cells (mTECs) play a crucial role in suppressing the onset of autoimmunity by eliminating autoreactive T cells and promoting the development of regulatory T cells in the thymus. Although mTECs undergo turnover in adults, the molecular mechanisms behind this process remain unclear. This study describes the direct and indirect roles of receptor activator of NF-κB (RANK) and CD40 signaling in TECs in the adult thymus.
View Article and Find Full Text PDFOrganoid generation from trophoblast stem cells highlights distinct roles for cytotrophoblasts and stem cells in organoid formation and expansion.
Placenta
December 2024
Department of Obstetrics, Gynaecology and Reproductive Science, School of Medicine, Faculty of Medical and Health Sciences, The University of Auckland, 85 Park Road, Grafton, Auckland, 1023, New Zealand. Electronic address:
Background: Organoids are stem-cell derived, self-organised, three-dimensional cultures that improve in vitro recapitulation of tissue structure. The generation of trophoblast organoids using primary placental villous digests (containing cytotrophoblasts and trophoblast stem cells (TSC)) improved high-throughput assessment of early trophoblast differentiation. However, the relative contributions of cytotrophoblasts and TSCs to trophoblast organoid growth and differentiation remain unclear, with implications for model interpretation.
View Article and Find Full Text PDFSingle-cell transcriptomics of rectal organoids from individuals with perianal fistulizing Crohn's disease reveals patient-specific signatures.
Sci Rep
October 2024
Department of Pediatrics & Pediatric Research Institute, Division of Pediatric Gastroenterology, Emory University School of Medicine & Children's Healthcare of Atlanta, Atlanta, GA, USA.
Article Synopsis
- Perianal fistulizing Crohn's disease is a severe condition with limited treatment options, especially affecting non-European populations, but the underlying genetic and cellular reasons are not well understood.
- This study investigated rectal organoids from patients with and without Crohn's disease, analyzing gene expression at a single-cell level to identify specific pathways and cell types linked to the disease.
- The findings revealed that organoids showed disease-related gene expression patterns and retained unique signatures related to gender and ancestry, indicating their potential for personalized research on Crohn's disease.
Single-Cell Transcriptomic Analysis Reveals Dynamic Cellular Processes in Corneal Epithelium During Wound Healing in Cynomolgus Monkeys.
Invest Ophthalmol Vis Sci
September 2024
State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou, China.
Article Synopsis
- The study investigates how corneal wound healing works by analyzing corneal limbal epithelial cell activities in cynomolgus monkeys through single-cell RNA sequencing.
- Researchers collected corneal limbal tissues at uninjured, 1-day, and 3-day healing stages to understand the transcriptional changes and cell migration involved in healing.
- The findings reveal nine distinct cell clusters, emphasizing the roles of different epithelial cells in healing, particularly highlighting the significance of transit amplifying cells in early healing through specific gene activation.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!