AI Article Synopsis
- Researchers developed an artificial zinc finger protein (AZP) technology to inhibit replication of DNA viruses, previously shown effective in plants, and applied it to human papillomavirus type 18 (HPV-18).
- Two AZPs, AZP(HPV)-1 and AZP(HPV)-2, were engineered to block the binding of the HPV-18 E2 protein to its replication origin, with AZP(HPV)-2 showing superior performance.
- In lab tests, both AZPs reduced viral DNA replication significantly, particularly AZP(HPV)-2, which effectively recognized and targeted the replication origin in mammalian cells, suggesting broad potential for AZP technology.
Article Abstract
Recently, we demonstrated that plant DNA virus replication was inhibited in planta by using an artificial zinc finger protein (AZP) and created AZP-based transgenic plants resistant to DNA virus infection. Here we apply the AZP technology to the inhibition of replication of a mammalian DNA virus, human papillomavirus type 18 (HPV-18). Two AZPs, designated AZP(HPV)-1 and AZP(HPV)-2, were designed by using our nondegenerate recognition code table and were constructed to block binding of the HPV-18 E2 replication protein to the replication origin. Both of the newly designed AZPs had much higher affinities towards the replication origin than did the E2 protein, and they efficiently blocked E2 binding in vitro. In transient replication assays, both AZPs inhibited viral DNA replication, especially AZP(HPV)-2, which reduced the replication level to approximately 10%. We also demonstrated in transient replication assays, using plasmids with mutant replication origins, that AZP(HPV)-2 could precisely recognize the replication origin in mammalian cells. Thus, it was demonstrated that the AZP technology could be applied not only to plant DNA viruses but also to mammalian DNA viruses.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1472147 | PMC |
| http://dx.doi.org/10.1128/JVI.01795-05 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The interactive effect of affectionate nicknames and streamer type on streamer attitude in E-commerce live streaming: the mediating role of psychological closeness.
Front Psychol
December 2024
Management School, Wuhan University of Science and Technology, Wuhan, China.
Background: With the widespread application of Artificial Intelligence technology in the field of E-commerce, human-machine relationships have attracted considerable attention within the field of psychology. Address forms, as crucial linguistic cues, have shown notable progress in advancing research on interpersonal relationships; however, a comprehensive understanding of the dynamics in interpersonal (or human-machine) relationships among interactors remains elusive. Therefore, based on Social Identity Theory, this paper explores the interactive effects and underlying mechanisms of affectionate nicknames and streamer type on streamer attitude in E-commerce live streaming, with consumers' perceptions of psychological closeness serving as the mediating mechanism.
View Article and Find Full Text PDFComputational modeling of fear and stress responses: validation using consolidated fear and stress protocols.
Front Syst Neurosci
December 2024
Universidade Federal de Goias, School of Electrical, Mechanical and Computer Engineering, Goiânia, Brazil.
Dysfunction in fear and stress responses is intrinsically linked to various neurological diseases, including anxiety disorders, depression, and Post-Traumatic Stress Disorder. Previous studies using in vivo models with Immediate-Extinction Deficit (IED) and Stress Enhanced Fear Learning (SEFL) protocols have provided valuable insights into these mechanisms and aided the development of new therapeutic approaches. However, assessing these dysfunctions in animal subjects using IED and SEFL protocols can cause significant pain and suffering.
View Article and Find Full Text PDFDevelopment of a Japanese version of the patient perceptions of deprescribing - Short form.
J Gen Fam Med
January 2025
Background: Deprescribing is a critical component of clinical practice, especially in geriatric medicine. Nevertheless, the attributes of patients who are prepared for, interested in, and could potentially benefit from deprescribing have not been well examined. The Patient Perceptions of Deprescribing (PPoD) evaluates patients' overall readiness for deprescribing and is complemented by an 11-item validated short form (SF-PPoD).
View Article and Find Full Text PDFMachine learning and molecular docking prediction of potential inhibitors against dengue virus.
Front Chem
December 2024
African Society for Bioinformatics and Computational Biology, Cape Town, South Africa.
Introduction: Dengue Fever continues to pose a global threat due to the widespread distribution of its vector mosquitoes, and . While the WHO-approved vaccine, Dengvaxia, and antiviral treatments like Balapiravir and Celgosivir are available, challenges such as drug resistance, reduced efficacy, and high treatment costs persist. This study aims to identify novel potential inhibitors of the Dengue virus (DENV) using an integrative drug discovery approach encompassing machine learning and molecular docking techniques.
View Article and Find Full Text PDFComparison of treatments for equine laryngeal hemiplegia using computational fluid dynamic analysis in an equine head model.
Front Vet Sci
December 2024
Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada.
Introduction: Computational fluid dynamics (CFD) is gaining momentum as a useful mechanism for analyzing obstructive disorders and surgeries in humans and warrants further development for application in equine surgery. While advancements in procedures continue, much remains unknown about the specific impact that different surgeries have on obstructive airway disorders. The objective of this study was to apply CFD analysis to an equine head inhalation model replicating recurrent laryngeal neuropathy (RLN) and four surgical procedures.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!