AI Article Synopsis
- * PFGE techniques can successfully separate DNA molecules over 10 megabase pairs, enabling the study of many previously uncharacterized fungal species.
- * This review highlights how PFGE-generated electrophoretic karyotypes can assist in understanding genome structure, identifying strains, and defining species boundaries.
Article Abstract
The separation of chromosome-size DNA molecules by pulsed-field gel electrophoresis (PFGE) has become a well-established technique in recent years. Although it has very wide-ranging applications, it made a real breakthrough for fungal genome analysis. Because of the small size of fungal chromosomes, their investigation was not possible earlier. Different PFGE approaches allowed the separation of DNA molecules larger than 10 megabase pairs in size, and electrophoretic karyotypes for numerous previously genetically uncharacterized fungal species could be established. This review discusses the applicability of these electrophoretic karyotypes for the investigation of genome structure, for strain identification and for species delimitation.
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| http://dx.doi.org/10.1556/AMicr.53.2006.1.7 | DOI Listing |
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