A simple manual rosetting method for absolute CD4+ lymphocyte counting in resource-limited countries.

Clin Vaccine Immunol

Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.

Published: May 2006

A CD4 monoclonal antibody which reacts with CD4+ lymphocytes but does not react significantly with monocytes was generated and used to develop a simple method for CD4 count. In a comparison with standard flow cytometry, high correlation was obtained. The developed method can be an alternative to flow cytometry for resource-limited countries.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1459645PMC
http://dx.doi.org/10.1128/CVI.13.5.598-601.2006DOI Listing

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