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Iodination of proteins by IPy2BF4, a new tool in protein chemistry. | LitMetric

Iodination of proteins by IPy2BF4, a new tool in protein chemistry.

Biochemistry

Proteomics Unit, Department of Experimental and Health Sciences, Universitat Pompeu Fabra, Dr. Aiguader 80, 08003 Barcelona, Spain.

Published: May 2006

AI Article Synopsis

Article Abstract

Iodination is a very useful method for protein characterization and labeling. However, derivatization chemistries used in most conventional iodination procedures may cause substantial alterations in protein structure and function. The IPy(2)BF(4) reagent [bis(pyridine)iodonium (I) tetrafluoroborate] has been shown to be an effective iodinating reagent for peptides. Herein we report the first application of IPy(2)BF(4) in protein iodination in an aqueous medium using three representative substrates: insulin, lysozyme, and the enzyme 1,3-1,4-beta-d-4-glucanohydrolase. Our results show that IPy(2)BF(4) has clear advantages over existing methods in that the reaction is quantitative, fast, and selective for the most accessible Tyr residues of a protein, and it preserves the functional integrity of the protein when moderate Tyr labeling levels are pursued.

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Source
http://dx.doi.org/10.1021/bi060103fDOI Listing

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