A human immunodeficiency virus type 1 (HIV-1)/HIV-2 antibody screening assay, the Genetic Systems HIV-1/HIV-2 PLUS O EIA, was compared to several established screening or confirmatory tests using an acute HIV seroconversion panel. The HIV-1/HIV-2 PLUS O EIA showed an improved sensitivity over all tested antibody screening methods, and detected antibody in 7 of 19 specimens found to be negative by a first-generation EIA but positive for the presence of HIV RNA.
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http://dx.doi.org/10.1128/JCM.44.5.1856-1858.2006 | DOI Listing |
Rev Inst Med Trop Sao Paulo
December 2024
Universidade de São Paulo, Faculdade de Medicina, Hospital das Clinicas, Laboratório de Investigação Médica (LIM-06), São Paulo, São Paulo, Brazil.
Using a panel study design, we aimed to estimate the seroconversion and seroreversion rates of anti-Strongyloides IgG antibodies from surveys carried out 11 months apart in a rural community in the Amazon Basin in Brazil. We used enzyme immunoassays to measure anti-Strongyloides IgG antibodies in 325 baseline plasma samples and 224 others that were collected 11 months later from residents in the agricultural settlement of Granada, Acre State. We observed anti-Strongyloides IgG antibodies in 21.
View Article and Find Full Text PDFVirol J
November 2024
Center of Clinical Laboratory Medicine, Zhongda Hospital, Southeast University, Nanjing, 210009, People's Republic of China.
Background: Human immunodeficiency virus (HIV) infection screening and diagnosis are critical to control the HIV epidemic. Testing for anti-HIV antibodies (Ab) and antigens (Ag) in blood samples is the first step to screen people who have been potentially exposed to the virus. This study aimed to evaluate the performance of the MAGLUMI HIV Ab/Ag Combi for detection of HIV antibodies and antigens.
View Article and Find Full Text PDFJ Clin Virol
December 2024
Abbott Diagnostics, Abbott Park, IL, USA. Electronic address:
Introduction: HCV antibody assays have been used to screen for HCV, but confirmation of acute infection is dependent on RNA or core antigen testing. The aim of the study was to compare the performance of five HCV test methods, including RNA testing, on a US emergency department population.
Methods: Clinical performance metrics were calculated on 708 consenting Johns Hopkins Emergency Department patients who self-reported an increased risk for HCV infection.
PLoS One
October 2024
Division of Global HIV & TB, Centers for Disease Control and Prevention, Atlanta, GA, United States of America.
Background: We developed the HIV Triplex multiplex bead assay to identify and serotype HIV infection with high sensitivity and specificity; and distinguish recent from long-term HIV-1 infections. It can facilitate accurate incidence estimation, while reducing the number of tests and blood collected, which is highly desirable for use in future studies and surveys. Using previously collected, treatment-naive longitudinal seroconversion HIV-1 positive panels and specimens from individuals infected for >12 months, we determined the assay's mean duration of recent infection (MDRI) and false-recency rate (FRR) respectively, at various mean fluorescent intensity (MFI) cutoffs.
View Article and Find Full Text PDFJ Proteome Res
September 2024
Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia.
Leptospirosis, a notifiable endemic disease in Malaysia, has higher mortality rates than regional dengue fever. Diverse clinical symptoms and limited diagnostic methods complicate leptospirosis diagnosis. The demand for accurate biomarker-based diagnostics is increasing.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!