Activity levels of UDP-glucose: (1,3)-beta-glucan (callose) synthase in microsomal membranes of pericarp tissue from tomato fruit (Lycoperisicon esculentum Mill, cv Rutgers) were determined during development and ripening. Addition of the phospholipase inhibitors O-phosphorylcholine and glycerol-1-phosphate to homogenization buffers was necessary to preserve enzyme activity during homogenization and membrane isolation. Enzyme activity declined 90% from the immature green to the red ripe stage. The polypeptide composition of the membranes did not change significantly during ripening. The enzyme from immature fruit was inactivated by exogenously added phospholipases A(2), C, and D. These results suggest that the decline in callose synthase activity during ontogeny may be a secondary effect of endogenous lipase action.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1080823 | PMC |
| http://dx.doi.org/10.1104/pp.96.2.664 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The effect of silicon supplementation and drought stress on the deposition of callose and chemical components in the cell walls of the Brassica napus roots.
BMC Plant Biol
December 2024
The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, Kraków, 30-239, Poland.
Background: Silicon has an important role in regulating water management in plants. It is deposited in cell walls and creates a mechanical barrier against external factors. The aim of this study was to determine the role of silicon supplementation in the synthesis and distribution of callose in oilseed rape roots and to characterize the modifications of cell wall structure of these organs after exposure to drought stress.
View Article and Find Full Text PDFMitigation of aluminum toxicity in rice seedlings using biofabricated selenium nanoparticles and nitric oxide: Synergistic effects on oxidative stress tolerance and sulfur metabolism.
Chemosphere
February 2025
Department of Botany, University of Gour Banga, Malda, 732103, West Bengal, India.
Biofabricated selenium nanoparticles (Se-NPs) and sodium nitroprusside-derived nitric oxide (NO) singly or in combination was evaluated to improve tolerance to aluminum (Al) stress in rice (Oryza sativa L. cv. Swarna Sub1).
View Article and Find Full Text PDFEffect of UV-B stress on olive (Olea europaea L.) pollen tubes: A study of callose plug deposition and male germ unit integrity.
Protoplasma
November 2024
Department of Life Sciences, University of Siena, Via Mattioli 4, 53100, Siena, Italy.
While UV-B radiation is beneficial to plant growth, it can also cause adverse effects. The pollen tube, a key component of plant reproduction with a tip growth mechanism, is an excellent cellular model for understanding how environmental stressors such as UV-B radiation affect plant cell growth. This research investigated the effect of UV-B on olive pollen both before and after germination.
View Article and Find Full Text PDFEffector FsMEP1 Contributes to Virulence by Disturbing Localization of Thiamine Thiazole Synthase ScTHI2 from Sugarcane.
Int J Mol Sci
November 2024
Guangxi Key Laboratory of Sugarcane Biology, Nanning 530004, China.
is a significant pathogenic fungus that causes sugarcane Pokkah Boeng. Proteins secreted by pathogenic fungi can be delivered into hosts to suppress plant immunity and establish infection. However, there is still much to be discovered regarding 's secreted effectors in overcoming plant immunity.
View Article and Find Full Text PDFEvaluation of Skin Color Supervision Genes in Chickpea Seeds by Multiomics.
Mol Biotechnol
November 2024
Institute of Horticulture, Xinjiang Academy of Agricultural Sciences, Urumqi, 830000, China.
Seed samples of two types of chickpea (Cicer arietinum Linn.), including variety A (NRCGR-4452) and variety B (local varieties), with different seed colors, were collected every five days for a total of four times during the seed development period. Non-targeted metabolome and transcriptome sequencing were conducted to identify differentially expressed genes and metabolites associated with chickpea seed coat color.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!