Genetic variability in the large and small subunits of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCase) in several Nicotiana species has been characterized by isoelectric focusing patterns. This heritable variation provides an opportunity to examine the functional role of each of these subunits. In this study, specifically designed RuBPCase enzymes composed of identical large subunits but different small subunits were constructed in vivo by interspecific hybridization between the species N. sylvestris, N. tabacum, N. glauca, N. glutinosa, N. plumbaginifolia, and N. tomentosiformis. Small subunit polypeptides were combined to form a sequence of one, two, three, and four polypeptides with the large subunit of N. sylvestris. Kinetic properties of these hybrid enzymes were compared. No differences in the specific activity of either carboxylation or oxygenation nor in K(m) values for ribulose 1,5-bisphosphate, CO(2), or O(2) were detected among the RuBPCase enzymes from the various interspecific hybrids. Likewise, the ratio of carboxylation to oxygenation was constant.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1066045 | PMC |
| http://dx.doi.org/10.1104/pp.71.2.404 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Side-by-Side Comparison of the In Vivo Performance of [Pb]Pb-DOTAMTATE and Other SSTR2-Targeting Compounds.
J Nucl Med
January 2025
Orano Med LLC, Plano, Texas.
There are numerous versions of octreotide and octreotate, including DOTAMTATE, DOTATATE, JR11, and lead-specific chelator (PSC)-PEG2-TOC. These peptides, which can be either analogs or antagonists, are used in nuclear medicine for diagnostic imaging or targeted radionuclide therapy of neuroendocrine tumors that are positive for somatostatin receptors (SSTRs). Despite their structural and targeting similarities, they have distinct properties and clinical uses.
View Article and Find Full Text PDFSynthesis of waterborne polyurethane-carboxymethyl chitosan cross-linked biodegradable bio-based porous materials for the adsorption of methylene blue.
Int J Biol Macromol
January 2025
State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China; Faculty of Light Industry, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China.
The development of green and cost-effective biomass adsorbents is necessary for removing large amounts of dyes from wastewater. In this study, polyurethane prepolymers were synthesized using polycaprolactone diol (OH-PCL-OH), isophorone diisocyanate, and 2,2-dihydroxymethylpropionic acid, which were subsequently dispersed in aqueous carboxymethyl chitosan (CMCS) solution to produce waterborne polyurethane (WPU)-CMCS porous materials. The adsorbent was characterized by scanning electron microscopy (SEM), Fourier transform infrared spectrometer (FT-IR), thermogravimetric (TGA) and mercury intrusion porosimetry (MIP).
View Article and Find Full Text PDFPhenylboronic acid functionalized magnetic ferroferric oxide nanoparticles and capillary electrophoresis for efficient isolation of flavonoid compounds from leaves of Lonicera japonica Thunb.
J Chromatogr A
January 2025
Department of Chemical Engineering, Jashore University of Science and Technology, Jashore 7408, Bangladesh.
Flavonoids are bioactive components in natural products, which possess anti-inflammatory, antibacterial, antioxidant, and cardiovascular protective properties. However, due to the complexity and low content of the components in these samples, developing rapid and sensitive methods for the isolation and extraction of flavonoids still remains a challenge in medical and food science. Herein, a 4-formylphenylboronic acid functionalized magnetic FeO nanomaterial (FeO@FPBA) was synthesized and applied as a sorbent of magnetic solid-phase extraction (MSPE) to covalently extract flavonoids from leaves of Lonicera japonica Thunb.
View Article and Find Full Text PDFImpact of high pressure impregnation and air drying on the quality of Dosidicus gigas slices.
Sci Rep
January 2025
Institute of Agricultural Engineering, Wroclaw University of Environmental and Life Sciences, Chełmońskiego 37/41, Wroclaw, 51-630, Poland.
Humboldt squid (Dosidicus gigas) is the most abundant cephalopod in the fishing industry, and its high nutritional and organoleptic properties make it a go-to food product for consumers. Therefore, developing new processing techniques seems imperative to minimize quality deterioration and provide products with appropriate characteristics. The study aimed to determine the effect of high-pressure impregnation (HPI) pretreatment on hot air-drying kinetics and the quality of Humboldt squid slices.
View Article and Find Full Text PDFKinetic and isothermal study of dye absorption using pre-treated natural fabrics using polyamine compounds.
Sci Rep
January 2025
Finishing of Cellulose-based Fibres Department, National Research Centre, Pretreatment and Textile Research and Technology Institute, 33 El-Behouth St. (former El-Tahrir str.), Dokki, P.O. 12622, Giza, Egypt.
The study examined the use of cationic polymers (Polyethyleneimine and chitosan) in treating fabrics like cotton, wool, and cotton/wool (70/30) to improve their dyeability and printability. The study examined factors such as dye concentration, time, and temperature for the dyeing process. Results showed that all dyed and printed fabrics treated with polyethyleneimine and chitosan increased color strength by significant percentages.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!