Radioimmune assay has been made of the ACTH content of the blood and adenohypophysis, as well as the content of vasopressin in the blood, median eminence, the posterior and intermediate hypophysis in 1, 3, 5, 7, and 20 days old rats and adult male rats. Comparison of the hormonal level in neurohaemal areas with that in the blood plasma suggests that transadenohypophyseal path of hypothalamic neurohormonal control of the adrenocortical function becomes essentially important only after formation of capillary plexus in the external zone of the median eminence.
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