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Isolation and translation of plant messenger RNA. | LitMetric

Isolation and translation of plant messenger RNA.

Plant Physiol

Department of Biology, Brandeis University, Waltham, Massachusetts 02154.

Published: July 1975

A fraction of the RNA species isolated from Lemna gibba G-3 consists of molecules with attached sequences of polyadenylic acid. This polyadenylic acid-containing fraction, separated from total RNA by adsorption onto oligothymidylic acid-cellulose, was shown to be mRNA by its ability to serve as template in a cell-free translation system derived from wheat germ. The products of translation were characterized by electrophoresis. This method permitted the comparison of mRNA from plants grown under different light conditions. Such plants were shown to possess qualitative and quantitative differences in their mRNA complements.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC541303PMC
http://dx.doi.org/10.1104/pp.56.1.88DOI Listing

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