A particulate enzyme system from Phaseolus aureus seedlings catalyzes the synthesis of alkali insoluble polysaccharide material from UDP-d-glucose. 80 to 90% of the d-glucose units are joined by beta-1,4 linkages, the remainder being combined by beta-1,3 linkages. It is not known whether the material is a single polysaccharide or a mixture.With a substrate concentration of 4 x 10(-3)m the enzyme system catalyzes the formation of alkali insoluble polysaccharide from UDP-d-glucose at a rate 23 times greater than that from GDP-d-glucose. The modifications introduced also increase the formation of cellulose from GDP-d-glucose to 3 times the rate previously reported.None of the glycosyl nucleotides containing bases other than guanine or uracil (adenine, cytosine, thymine) served as substrate for the production of alkali insoluble polysaccharide with this enzyme system. dUDP-d-glucose could also not serve as substrate.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1086706 | PMC |
| http://dx.doi.org/10.1104/pp.42.9.1219 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Fully biosourced amphiphilic block copolymer from tamarind seed xyloglucan and solanesol: synthesis, aqueous self-assembly, and drug encapsulation.
Carbohydr Polym
March 2025
Faculty of Engineering, Hokkaido University, Sapporo 060-8628, Japan. Electronic address:
This study aims to explore the development of natural bio-based amphiphilic block copolymers for drug delivery applications. We investigated block copolymers derived from tamarind seed xyloglucan and solanesol, focusing on their synthesis, structural analysis, aqueous self-assembly, and drug encapsulation. Specifically, xyloglucan hydrolysate segments with number-average degrees of polymerization (DPs) of between 8 and 44 (XOS, XMS, XMS, XMS, and XMS) were used as the hydrophilic blocks, whereas plant-sourced solanesol was selected as the hydrophobic segment.
View Article and Find Full Text PDFCombined use of steam explosion, alkali, and microbial methods improving the yield, structure and properties of soluble dietary fiber from bamboo shoot shells.
Food Chem
January 2025
College of biological and food engineering, Anhui Polytechnic University, 241000 Wuhu, China; Wuhu Green Food Industry Research Institute Co., Ltd., 241000 Wuhu, China; Wuhu Hight Biotechnology Co., Ltd, 241000 Wuhu, China; Anhui Engineering Laboratory for Industrial Microbiology Molecular Breeding, 241000 Wuhu, China. Electronic address:
Developing an effective method for extracting soluble dietary fiber (SDF) from bamboo shoot shell (BSS) is of great significance for the resource utilization of BSS. Here, we proposed the combinational strategy of steam explosion (SE), alkaline extraction (AE), and microbial extraction (ME) to enhance BSS-SDF yield. The highest yield of 28.
View Article and Find Full Text PDFThe Impact of Cooking on Antioxidant and Enzyme Activities in Ruichang Yam Polyphenols.
Foods
December 2024
Jiangxi Ecological Chemical Engineering Technology Research Center, Jiujiang University, Jiujiang 332005, China.
In this study, the total polyphenol content (TPC), total flavonoid content (TFC), and biological activity of yam polyphenols (including free phenolics, conjugated phenolics, and bound phenolics) were investigated during home cooking. Polyphenol components were preliminary detected in raw yam by HPLC, including 2, 4-dihydroxybenzoic acid, syringic acid, vanillic acid, 4-coumaric acid, and sinapic acid. TPC and TFC of soluble conjugated polyphenols were the main phenolic compounds in Ruichang yam.
View Article and Find Full Text PDFStructure and strain specificity for polysaccharides from king oyster mushroom (Pleurotus eryngii) fruiting bodies.
Int J Biol Macromol
January 2025
Department of Crop Production, Czech University of Life Sciences Prague, Czech Republic.
Article Synopsis
- The study examines two Tunisian strains of king oyster mushroom (P. eryngii) for their polysaccharide content and compares them to a Korean strain.
- Polysaccharides were extracted using hot water and sodium hydroxide, revealing glucans as the primary component, with notable differences in water-solubility between the Tunisian strains and the reference strain.
- The findings suggest that the Tunisian strains may offer higher nutritional value due to better protein availability and are promising candidates for culinary use in North Africa.
[ADMET Analysis of Amorphous β-Carotene and Its Usefulness Evaluation].
Yakugaku Zasshi
January 2025
Graduate School of Pharmaceutical Sciences, Osaka University.
In recent years, functional foods have attracted increasing attention due to growing health consciousness. When functional food ingredients are poorly water-soluble, they largely fail to be absorbed due to their low solubility in the digestive tract, limiting their ability to exert their functions. To develop poorly water-soluble compounds into viable functional food ingredients, it is important to increase their gastrointestinal absorption so that they can fully exert their functions, and to ensure their safety and efficacy through ADMET research.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!