AI Article Synopsis

  • - KIF14 is a motor protein linked to poor outcomes in breast cancer; it accumulates during cell division and is found at spindle poles and midbodies in mitotic cells.
  • - Silencing KIF14 using RNA interference (RNAi) leads to failures in cell division, resulting in cells with multiple nuclei and increased apoptosis (cell death).
  • - The study shows that how effectively KIF14 is silenced impacts the types of problems that arise during different stages of the cell cycle, highlighting its role in cell division regulation.

Article Abstract

KIF14 is a microtubule motor protein whose elevated expression is associated with poor-prognosis breast cancer. Here we demonstrate KIF14 accumulation in mitotic cells, where it associated with developing spindle poles and spindle microtubules. Cells at later stages of mitosis were characterized by the concentration of KIF14 at the midbody. Time-lapse microscopy revealed that strong RNA interference (RNAi)-mediated silencing of KIF14 induced cytokinesis failure, causing several rounds of endoreduplication and resulting in multinucleated cells. Additionally, less efficacious KIF14-specific short interfering RNAs (siRNAs) induced multiple phenotypes, all of which resulted in acute apoptosis. Our data demonstrate the ability of siRNA-mediated silencing to generate epiallelic hypomorphs associated with KIF14 depletion. Furthermore, the link we observed between siRNA efficacy and phenotypic outcome indicates that distinct stages during cell cycle progression are disrupted by the differential modulation of KIF14 expression.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1488988PMC
http://dx.doi.org/10.1128/MCB.26.10.3853-3863.2006DOI Listing

Publication Analysis

Top Keywords

cell cycle
8
cycle progression
8
cytokinesis failure
8
kif14
7
rna interference-mediated
4
interference-mediated silencing
4
silencing mitotic
4
mitotic kinesin
4
kinesin kif14
4
kif14 disrupts
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!