Aim: To investigate whether insulin exerts protective effects against hepatocytes injury indirectly induced by LPS and if so, the mechanism involved.
Methods: Kupffer cells and hepatocytes were isolated from male Sprague-Dawley rats by pronasecollagenase perfusion and cultured. Kupffer cells were stimulated by lipopolysaccharide (LPS) or LPS+insulin for 4 h, the supernatant of different groups were collected to detect TNF-alpha and IL-10 by ELISA and to stimulate the primary cultured rat hepatocytes with or without anti-TNF-alpha antibody. After the hepatocytes were stimulated for 12 h, cell survival rate and injury indexes were detected.
Results: (1) The supernatant of LPS-activated Kupffer cells caused significant hepatocytes injury. Treatment with insulin produced a potent protective effect as evidenced by decreased ALT and AST levels (P<0.01, n=8) and improved hepatocytes survival rate (P<0.01, n=8). (2) Compared with the LPS group, insulin significantly decreased TNF-alpha secretion (P<0.01, n=8) and increased IL-10 secretion (P<0.05, n=8) from Kupffer cell stimulated by LPS. (3) Incubating hepatocytes with anti-TNF-alpha antibody attenuated hepatocytes injury induced by the supernatant of LPS-activated Kupffer cells. But treatment with anti-TNF-alpha antibody and insulin did not exert additive effect compared with treatment with insulin alone.
Conclusion: Insulin has protective effects on rat hepatocytes injury indirectly induced by LPS, which is associated with suppression of the pro-inflammatory cytokine TNF-alpha and increment of the anti-inflammatory cytokine IL-10 both from Kupffer cells.
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Poult Sci
January 2025
College of Animal Science and Technology, Northwest A&F University, Yangling 712100 Shaanxi, PR China. Electronic address:
DHAV-3 is one of the main causative agents of duck viral hepatitis (DVH), an acute and highly lethal infectious disease in duck industry. However, the understanding of the pathogenesis of this virus in ducklings is limited. To dissect the molecular characteristics associated with pathobiology of ducklings to DHAV-3, we applied single-cell RNA-sequencing approach to profile the transcriptome of 1.
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Department of Internal Medicine III, University Hospital RWTH Aachen, Pauwelsstraße 30, 52074, Aachen, Germany.
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Jiangsu Institute of Hematology, National Clinical Research Center for Hematologic Diseases, NHC Key Laboratory of Thrombosis and Hemostasis, The First Affiliated Hospital of Soochow University, Suzhou, China.
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University of California, San Diego, La Jolla, California, United States of America.
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Department of Forest Ecology and Protection, Kyungpook National University, Sangju 37224, Republic of Korea.
The cellular characteristics of the opportunistic fungal pathogen Cryptococcus species were investigated in the infected liver of an immunocompetent host using transmission electron microscopy (TEM). With no records of immunodeficiency, the 3-year-old female patient displayed a high-grade fever, lethargy, and increasing jaundice. TEM analysis revealed the presence of round yeast cells in the patient's liver.
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