The Sherlock Mycobacteria Identification HPLC system correctly identified to the species level 61 (67.8%) of 90 isolates growing on solid media, and 73 (45.3%) of 161 isolates directly from positive VersaTREK Myco bottles. When these data were re-analysed with a revised database, correct identifications increased to 91.1% and 83.2%, respectively. All Mycobacterium tuberculosis isolates were identified correctly, regardless of the inoculum source or database used. The use of the revised database with isolates obtained directly from positive VersaTREK Myco bottles allows the identification of most isolates within clinically relevant time-frames.
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Pathol Biol (Paris)
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Laboratoire de microbiologie, hôpital Emile-Muller, centre hospitalier de Mulhouse, 20, avenue du Docteur-Laennec, 68070 Mulhouse cedex, France.
Objectives: Our laboratory of microbiology use the VersaTREK/ESP Culture System II for the isolation of mycobacteria. In this study, we evaluate this system for the analysis carried out between April 2009 and March 2010.
Method: The Myco bottles are supplemented with growth supplements and an antimicrobial agent solution prior to inoculation with the specimen.
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Department of Laboratories, St Vincent's Hospital-Manhattan, 153 W. 11th Street, New York, NY 10011, USA.
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