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The Etiology of Intraocular Lens Dislocation and Changes in Intraocular Pressure After Intrascleral Intraocular Lens Fixation Surgery.
Cureus
December 2024
Ophthalmology and Visual Science, Chiba University Graduate School of Medicine, Chiba, JPN.
Objectives This study aimed to identify the etiology and the direction of dislocation of the natural crystalline lens or intraocular lens (IOL) in IOL intrascleral fixation surgery and to determine the change in intraocular pressure (IOP) after surgery. Methods We retrospectively investigated the diagnosis, direction of lens and IOL dislocation, and IOP before and after surgery (preoperatively and one day, one week, and one month postoperatively) in 236 eyes from 228 patients who underwent IOL intrascleral fixation at Chiba University Hospital between February 2015 and September 2020. Results IOL intrascleral fixation was performed in 48 (20.
View Article and Find Full Text PDFImpact of Timing Surgical Fixation on Complications and Functional Outcomes in Ankle Fractures.
Cureus
December 2024
Trauma and Orthopaedics, Wythenshawe Hospital, Manchester University NHS Foundation Trust, Manchester, GBR.
Introduction: This study aimed to assess whether adherence to the British Orthopaedic Association Standards for Trauma (BOAST) and National Institute for Health and Care Excellence (NICE) guidelines for ankle fractures is associated with reduced complication rates and improved functional outcomes. Methods: A retrospective analysis was conducted of all patients who underwent surgical fixation for ankle fractures in August 2023 to January 2024 from an acute hospital. Statistical analyses were performed using IBM SPSS Statistics software, version 29 (IBM Corp.
View Article and Find Full Text PDFPrevalence and histopathological analysis of cystic echinococcosis in ruminants of District Narowal, Pakistan: focus on pulmonary involvement.
Rev Bras Parasitol Vet
December 2024
Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
A total of 384 animals (sheep, goat, cattle, and buffalo) were examined for the presence of hydatid cysts only in the lungs. The lung tissue samples associated with the hydatid cyst were collected immediately after slaughter, followed by fixation in 10% formalin. The fixed tissue was subjected to paraffin embedding technique.
View Article and Find Full Text PDFFormalin-free tissue embedding is less hazardous and results in better DNA quality.
PLoS One
January 2025
Isala Pathology, Zwolle, The Netherlands.
Disease diagnosis, prognosis, and therapy choice progressively rely on good-quality Deoxyribonucleic acid (DNA) for molecular analysis. As tissue processing is routinely performed worldwide with ancient techniques using toxic and DNA-damaging formaldehyde, the quality of DNA isolated from embedded tissues used for diagnostics is poor. We used a novel formalin-free tissue embedding method to process tissues and show that, after 5 years, DNA quality is superior to formalin fixation.
View Article and Find Full Text PDFProtocol for the isolation of brain microvessels and visualization of RNA fluorescence in mice and humans.
STAR Protoc
January 2025
University of Miami Miller School of Medicine, Department of Biochemistry and Molecular Biology, Miami, FL, USA; Institute of Physiotherapy and Health Sciences, the Blood-Brain Barrier Research Center, the Jerzy Kukuczka Academy of Physical Education, Katowice, Poland. Electronic address:
Here, we present a protocol for isolating microvessels from fresh or snap-frozen brain tissue from mice and humans, followed by visualization of RNA utilizing RNAscope hybridization for quantification of mRNA. We describe the steps for sample preparation and isolation, fixation, and hybridization. This protocol was specifically designed to integrate with RNAscope in situ hybridization.
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