AI Article Synopsis
- Prodigiosin (PG) is a red-pigmented antibiotic from bacteria that has the ability to suppress immune responses and induce cell death (apoptosis).
- Researchers investigated how PG triggers apoptosis by analyzing changes in protein expression in MCF-7 cancer cells resistant to a specific chemotherapy drug.
- Six key proteins linked to PG-induced apoptosis were identified, which play roles in cell defense, DNA repair, and overall cellular organization, providing new insights into PG's potential as an anticancer treatment.
Article Abstract
Prodigiosin (PG) is a bacterial, red-pigmented antibiotic with immunosuppressive and apoptotic activities. To better understand its mechanisms of action, we tried to identify proteins associated with apoptosis induced by PG. For this purpose, the variation of protein expression on exposure to apoptotic concentrations of PG was examined, by high-resolution two-dimensional gel electrophoresis (2D-E), in the MCF-7 cancer cell line resistant to mitoxantrone (MCF-7-MR). Six PG apoptosis-associated protein spots were further characterized by complementary peptide mass fingerprinting and tandem mass spectrometry data obtained on a matrix-assisted laser desorption ionization-time-of-flight/time-of-flight (MALDI-TOF/TOF) mass spectrometer. The proteins identified were involved in various cellular functions, including cell defence, DNA repair and cellular organization. Our data provide novel information on cell response to PG, a new apoptotic drug with interesting anticancer activity.
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| http://dx.doi.org/10.1007/s10637-006-7774-8 | DOI Listing |
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