Proprotein convertase furin is preferentially expressed in T helper 1 cells and regulates interferon gamma.
Blood
MIIB-NIAMS-NIH, Bldg 10, Rm 9N262, 10 Center Dr, Bethesda, MD 20892, USA.
Published: August 2006
Interleukin 12 (IL-12) is a major inducer of interferon gamma (IFN-gamma) and the principal mediator of T helper 1 (Th1) differentiation. To identify IL-12-regulated genes, which might contribute to Th1 differentiation and IFNG regulation, we employed microarray analysis. Surprisingly, a ubiquitously expressed proprotein convertase (PC), furin, was one of the most consistently IL-12-induced genes in T cells, and among PCs was the only one regulated by this cytokine. Furin was preferentially expressed in differentiated Th1 cells in a Stat4-dependent manner. Expression of furin enhanced IFN-gamma secretion, whereas inhibition of furin interfered with IFN-gamma production. Thus, we conclude that IL-12 induction of furin might represent a new aspect of IFN-gamma regulation and control of Th1 differentiation.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1895858 | PMC |
| http://dx.doi.org/10.1182/blood-2005-09-3824 | DOI Listing |
Similar Publications
Expressing Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Single-Chain Antibody Can Inhibit PRRSV Replication and Change the Intestinal Flora Structure of Piglets.
Int J Mol Sci
March 2025
College of Veterinary Medicine, Jilin Agricultural University, Changchun 130012, China.
Porcine reproductive and respiratory syndrome (PRRS) is an infectious disease that can cause reproductive disorders in sows and affect the breathing of piglets, seriously endangering pig breeding worldwide. In this study, NC8 was used as the expression delivery vector of foreign proteins, and a single-chain antibody was designed based on an mAb-PN9cx3 sequence. Three recombinant strains of , namely, NC8/pSIP409-pgsA'-PN9cx3-scFV(E), NC8/pSIP409-pgsA'-PN9cx3-HC(E), and NC8/pSIP409-pgsA'-PN9cx3-LC(E), were successfully constructed.
View Article and Find Full Text PDFBlood transcriptome signature as indicator and predictor for efficacy of abrocitinib in treatment of atopic dermatitis.
J Invest Dermatol
March 2025
Department of Dermatology, Shanghai Institute of Dermatology, National Clinical Research Center for Aging and Medicine, Huashan Hospital, Fudan University, Shanghai, 200040, P. R. China. Electronic address:
Atopic dermatitis (AD) patients exhibit significant blood transcriptome alterations, reflecting systemic inflammation. The effects of abrocitinib, a Janus kinase 1 inhibitor, on the blood transcriptome of AD remain unclear. This study aim to investigate abrocitinib's effects on the blood transcriptome in AD patients and identify transcriptomic predictors of treatment efficacy.
View Article and Find Full Text PDFCircular RNAs (circRNAs) are endogenous non-coding RNA molecules that form covalently closed molecular loops. By regulating gene expression, circRNAs are known to play crucial roles in the development and progression of various diseases, including autoimmune, neoplastic, and neurological disorders. In this study, we examined the expression of circSnx5 in inflamed CNS tissue at different stages of experimental autoimmune encephalitis (EAE), an animal model for multiple sclerosis (MS), as well as in T cells that were activated and differentiated into different T helper phenotypes (Th1, Th17, Treg).
View Article and Find Full Text PDFEffects of Th1/Th17 and Th2 cytokines on lipid metabolism in differentiated keratinocytes.
Front Physiol
February 2025
Laboratory of Cutaneous Physiopathology, San Gallicano Dermatological Institute - IRCCS, Rome, Italy.
Introduction: Abnormalities of keratinocyte differentiation and impairment of permeability barrier are features of inflammatory skin diseases driven by Th1/Th17 and Th2 immune response, such as psoriasis and atopic dermatitis. We aimed at identifying the signature of the Th1/Th17 and Th2 environments on keratinocytes, focusing on the expression of genes involved in the lipid metabolism and profiles of abundance of lipid metabolites.
Methods: Human immortalized keratinocytes in prodifferentiative conditions induced by increasing calcium concentration, and 3D epidermal equivalents were treated with mixtures either of TNF-α and IL-17A plus Th1-related cytokines (IL-1α, IL-6) or of Th2 cytokines (IL-4, IL-13).
Peripheral immune profiling highlights a dynamic role of low-density granulocytes in myasthenia gravis.
J Autoimmun
March 2025
Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing, China. Electronic address:
Background: Myasthenia gravis (MG) is an autoimmune neuromuscular disease marked by dysregulation of several immune cell populations. Here we explored peripheral immune landscape, particularly the role of low-density granulocytes (LDGs).
Methods: Single-cell and bulk RNA sequencing analyzed peripheral immune cells from MG patients pre- (n = 4) and after treatment (n = 2), as well as healthy controls (n = 3).
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!
© LitMetric 2025. All rights reserved.