Induction of interleukin-8 expression in porcine peripheral blood mononuclear cells by trans10-cis12 conjugated linoleic acid.

The chemotaxis of porcine peripheral blood polymorphonuclear cells (PMN) was enhanced by culture supernatant from peripheral blood mononuclear cells (PBMC) treated with trans10-cis12 (10t-12c) conjugated linoleic acid (CLA) or by porcine recombinant (pr) interleukin (IL)-8. To identify this chemotactic inducing factor, the culture supernatant from 10t-12c CLA treated PBMC was partially purified by gel filtration. Only fraction 9 contained enhanced PMN chemotactic activity. The chemotactic activity of fraction 9 was inhibited by an anti-prIL-8 polyclonal antibody (pAb). Porcine IL-8 mRNA expression analyzed by a reverse transcriptase-polymerase chain reaction (RT-PCR) assay was increased in PBMC by the addition of 10t-12c CLA. This study strongly suggests that the immunoenhancing effect of CLA on the chemotactic response of porcine PMN is mediated through IL-8 produced by CLA treated PBMC.