Objective: To study the fungi isolated from Cordyceps sobolifera and its fermentation culture.

Methods: The fungi was isolated and identified by its hypha and spores. Three liquid media were used in the culture.

Results: Pure culture was gained and the fungi was identified to be Paecilomyces cicadae. The fungi can grow best in liquid media: egg 110 g + silkworm powder 30 g + VB1 2 tahlets + MgSO4 x 7H2O 0.5 g + K2HPO4 1 g + H2O 1000 ml, in which every litre can produce 135 g wet hypha after cultured 4 d.

Conclusion: Paecilomyces cicadae from Cordyceps sobolifera can be cultured in liquid media.

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