AI Article Synopsis
- PDGF is implicated in disorders like atherosclerosis and cancer, but its molecular structure and receptor binding sites are not well understood.
- Specific amino acids in the PDGF-B chain, arginine 27 and isoleucine 30, are crucial for receptor affinity and cell activation; mutations here can cause defects.
- Studies using circular dichroism and fluorescence spectroscopy indicate that these mutations do not change the overall structure of PDGF.
Article Abstract
PDGF may be involved in the pathogenesis of a variety of disorders including atherosclerosis and certain types of cancer. There is currently little understanding of the molecular structure of PDGF and of the critical amino acid residues involved in receptor binding and cell activation. Two such PDGF-B chain residues, arginine 27 and isoleucine 30, have been identified by a site-directed mutagenesis programme. Substitutions in these positions can lead to PDGF mutants defective in both receptor affinity and cell activation as judged by displacement of [125I]PDGF-BB, mitogenic assay and inositol lipid turnover. Circular dichroism and fluorescence spectroscopy show that such mutations do not disrupt the structure of PDGF.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC453161 | PMC |
| http://dx.doi.org/10.1002/j.1460-2075.1991.tb04988.x | DOI Listing |
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