An important property of the bacterial surface is its conductivity. To obtain reliable conductivity values, it is essential to handle the cells as gently as possible during the measurement procedure. We have developed a method where a standard conductivity meter is used in combination with cross-flow filtration, thus avoiding repeated centrifugation and resuspension. With this method, the conductivity of Bacillus subtilis was determined to be 7,000 microS/cm, which is a deviation from previously published data by almost an order of a magnitude.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s10529-006-0021-8 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Dual-Stage Cross-Flow Filtration: Integrated Capture and Purification of Virus-Like Particles.
Biotechnol Bioeng
December 2024
Institute of Process Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
Virus-like particles (VLPs) are a versatile technology for the targeted delivery of genetic material through packaging and potential surface modifications for directed delivery or immunological issues. Although VLP production is relatively simple as they can be recombinantly produced using microorganisms such as Escherichia coli, their current downstream processing often relies on individually developed purification strategies. Integrating size-selective separation techniques may allow standardized platform processing across VLP purification.
View Article and Find Full Text PDFPermeability-selectivity trade-off for a universal leaky channel inspired by mobula filters.
Proc Natl Acad Sci U S A
December 2024
Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139.
Mobula rays have evolved leaf-shaped filter structures to separate food particles from seawater, which function similarly to industrial cross-flow filters. Unlike cross-flow filtration, where permeability and selectivity are rationally designed following trade-off analyses, the driving forces underlying the evolution of mobula filter geometry have remained elusive. To bridge the principles of cross-flow and mobula filtration, we establish a universal framework for the permeability-selectivity trade-off in a leaky channel inspired by mobula filters, where permeability and selectivity are characterized by the pore-scale leaking rate and the cut-off particle size, respectively.
View Article and Find Full Text PDFHeterogeneous Covalent Organic Framework Membranes Mediated by Polycations for Efficient Ions Separation.
Adv Sci (Weinh)
December 2024
Zhejiang International Joint Laboratory of Advanced Membrane Materials & Processes, Ningbo Institute of Materials Technology & Engineering, Chinese Academy of Sciences, Ningbo, 315201, China.
Precise ions sieving at angstrom-scale is gaining tremendous attention thanks to its significant impact at the water-energy nexus. Herein, a novel polycation-modulated interfacial polymerization (IP) strategy is developed to prepare a heterogeneously charged covalent organic frameworks (COFs) membrane. Cationic poly(diallyldimethylammonium chloride) (PDDA) regulates the growth and assembly of anionic COFs nanosheets, which thus provides a negative, smooth top surface and positive, rough bottom surface, indicating the presence of heterogeneously charged angstrom-scale channels through the membrane.
View Article and Find Full Text PDFUltrahigh-throughput cross-flow filtration of solution-processed 2D materials enabled by porous ceramic membranes.
Mater Horiz
November 2024
Department of Materials Science and Engineering, Northwestern University, Evanston, Illinois 60208, USA.
Article Synopsis
- Printed electronics is a game-changing technology used in various applications such as sensors, displays, and wearable devices, utilizing 2D materials for their excellent properties.
- Traditional methods for producing 2D electronic inks, like centrifugation, are time-consuming and inefficient, while newer methods face challenges due to low concentration requirements.
- The presented study introduces a continuous flow system using advanced ceramic membranes that enhance processing efficiency, significantly reduce environmental impact, and lower production costs for high-quality printable inks.
Ultra-permeable silk-based polymeric membranes for vacuum-driven nanofiltration.
Nat Commun
October 2024
Department of Civil Engineering, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.
Article Synopsis
- - Nanofiltration (NF) membranes traditionally come in spiral-wound modules, which can lead to high costs and operational challenges due to pressure issues.
- - A new silk-based membrane has been developed that features a unique crumpled design, achieving high water permeance and contaminant rejection rates under both traditional and vacuum-driven systems.
- - This innovative membrane could significantly reduce energy consumption and greenhouse gas emissions in water treatment processes, making it a promising alternative for practical industrial applications.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!