Objective: Production and characterization the polyantibodies against MCYST-LR.
Methods: microcystin-LR (MC-LR) was conjugated to KLH, then immune the rabbit by the routine method. Purify the antiserum by centrifugal and saturated sulfuric ammonium precipitated methods. Count the affinity constant and measure the titers, IC50 Value and the sensitivity.
Results: The titers of the antiserum is over 25600. IC50 value is about from 0.1 ng/ml to 1 ng/ml. The across-reactivity ratio to MC-LW and MC-LR is about from 0.1% to 20%.
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[Production purification and characterization of polyantibodies against microcystin-LR].
Wei Sheng Yan Jiu
January 2006
School of Food Science of Southern Yangtze University, Wuxi 214036, China.
Objective: Production and characterization the polyantibodies against MCYST-LR.
Methods: microcystin-LR (MC-LR) was conjugated to KLH, then immune the rabbit by the routine method. Purify the antiserum by centrifugal and saturated sulfuric ammonium precipitated methods.
[Preparation and characterization of immunogen against microcystin LR].
Wei Sheng Yan Jiu
November 2005
Jiangsu Institute of Microbiology, Wuxi 214063, China.
Objective: To prepare Microcystins-LR (MC-LR) conjugates with the carrier protein for developing a new immune assay for MC-LR.
Methods: MC-LR was coupled to BSA or KLH by "activated ester" and "water-soluble carbodimide". Measure the combine ratio of MC-LR with KLH or BSA by the UV scanning or the Bradford's method.
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