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| http://dx.doi.org/10.1073/pnas.57.2.423 | DOI Listing |
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DNA binding of an RNA helicase bacterial transcription terminator.
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The bacterial transcription terminator Rho is a hexameric ATP-dependent RNA helicase that dislodges elongating RNA polymerases. It has an N-terminal primary RNA binding site (PBS) on each subunit and a C-terminal secondary RNA binding site at the central channel. Here, we show that Rho also binds to linear longer double-stranded DNAs (dsDNA) and the circular plasmids non-specifically using its PBS.
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Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
Micron-scale colloidal particles with short-ranged attractions, e.g., colloids functionalized with single-stranded DNA oligomers, have emerged as a powerful platform for studying colloidal self-assembly phenomena with the long-term goal of identifying routes for metamaterial fabrication.
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Chemically modified nucleic acid molecules have been developed as oligonucleotide therapeutics, and its assay is critical in quality assurance. The common DNA/RNA quantification method using UV-260 nm can lack accuracy because of structure modifications and the possible formation of higher-order structure (HOS). Additionally, process-associated water and counterions affect the accuracy in gravimetric analysis.
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Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, Indiana, USA. Electronic address:
Article Synopsis
- SARS-CoV-2's nucleocapsid protein (N) packages viral RNA and forms structures in infected cells through a process called liquid-liquid phase separation (LLPS), which is essential for viral functions like replication and immune evasion.
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Light- and Temperature-Controlled Hybridization, Chiral Induction and Handedness of Helical Foldamers.
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Univ Angers, CNRS, MOLTECH-ANJOU, F-49000, Angers, France.
Helical foldamers have attracted much attention over the last decades given their resemblance to certain biomacromolecules and their potential in domains as different as pharmaceutics, catalysis and photonics. Various research groups have successfully controlled the right- or left- handedness of these oligomers by introducing stereogenic centers through covalent or non-covalent chemistry. However, developing helical structures whose handedness can be reversibly switched remains a major challenge for chemists.
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