AI Article Synopsis
- Nsp15, a non-structural protein from the SARS virus, has been identified as a uridine-specific endoribonuclease crucial for viral replication and transcription.
- The full-length Nsp15 protein, consisting of 346 amino acids, was successfully cloned and expressed in E. coli, where it was purified with an N-terminal hexahistidine tag.
- Crystallization of Nsp15 resulted in the formation of small cubic crystals, which belong to specific space groups and were analyzed at a maximum resolution of 2.7 angstroms.
Article Abstract
The non-structural protein Nsp15 from the aetiological agent of SARS (severe acute respiratory syndrome) has recently been characterized as a uridine-specific endoribonuclease. This enzyme plays an essential role in viral replication and transcription since a mutation in the related H229E human coronavirus nsp15 gene can abolish viral RNA synthesis. SARS full-length Nsp15 (346 amino acids) has been cloned and expressed in Escherichia coli with an N-terminal hexahistidine tag and has been purified to homogeneity. The protein was subsequently crystallized using PEG 8000 or 10 000 as precipitants. Small cubic crystals of the apoenzyme were obtained from 100 nl nanodrops. They belong to space group P4(1)32 or P4(3)32, with unit-cell parameters a = b = c = 166.8 angstroms. Diffraction data were collected to a maximum resolution of 2.7 angstroms.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2222560 | PMC |
| http://dx.doi.org/10.1107/S1744309106009407 | DOI Listing |
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