AI Article Synopsis
- Microcin C is a heptapeptide with a modified adenosine monophosphate that effectively inhibits bacterial cell growth.
- It targets translation, impacting the process of protein synthesis, although the exact mechanism was previously unclear.
- The degradation of Microcin C inside sensitive cells produces a byproduct that notably impairs translation by obstructing aspartyl-tRNA synthetase function.
Article Abstract
Microcin C is a ribosome-synthesized heptapeptide that contains a modified adenosine monophosphate covalently attached to the C-terminal aspartate. Microcin C is a potent inhibitor of bacterial cell growth. Based on the in vivo kinetics of inhibition of macromolecular synthesis, Microcin C targets translation, through a mechanism that remained undefined. Here, we show that Microcin C is a subject of specific degradation inside the sensitive cell. The product of degradation, a modified aspartyl-adenylate containing an N-acylphosphoramidate linkage, strongly inhibits translation by blocking the function of aspartyl-tRNA synthetase.
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| http://dx.doi.org/10.1074/jbc.M513174200 | DOI Listing |
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