[Determination of four components in root of Saposhnikovia divaricata by HPLC gradient elution].

Zhongguo Zhong Yao Za Zhi

Institute of Chinese Materia Media, China Academy of Chinese Medical Sciences, Beijing 100700, China.

Published: February 2006

Objective: To develop an HPLC method for determination of components in root of Saposhnikovia divaricata.

Method: The separation of four components was achieved on a reversed-phase Alltima C18 column with MeOH-H2O gradient elution at a flow rate of 1.0 mL x min(-1).

Result: Six samples were determined and there is no distinct difference between the contents determined by the new and old methods.

Conclusion: The new method is more simple and convenient than the old method.

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