AI Article Synopsis
- LEF-4 is a crucial subunit of the baculovirus RNA polymerase, necessary for late gene expression in the viral life cycle.
- Silencing LEF-4 in infected cells halts structural gene expression, indicating its role is specifically important for later stages of viral replication.
- Experiments show that without LEF-4, viral progeny cannot be produced, but co-transfecting with a normal LEF-4 plasmid restores replication, highlighting the importance of its mRNA capping function.
Article Abstract
Baculovirus lef-4 encodes one subunit of the viral RNA polymerase. Here, we demonstrate the essential nature of LEF-4 by RNA interference and bacmid knockout technology. Silencing of LEF-4 in wild-type virus-infected cells suppressed expression of structural genes, while early expression was unaffected, demonstrating its essential role in late gene expression. After transfection of insect cells with lef-4 mutant bacmid, no viral progeny was produced, further defining its central role in infection. Cotransfection with wild-type lef-4 plasmid restored normal replication, but plasmid encoding a guanyltransferase-deficient version failed to rescue. These results emphasize the importance of the mRNA capping function of LEF-4.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1440449 | PMC |
| http://dx.doi.org/10.1128/JVI.80.8.4168-4173.2006 | DOI Listing |
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