AI Article Synopsis
- A new method using capillary electrophoresis with UV detection has been developed for the separation and direct detection of peroxynitrite and its degradation products, nitrite and nitrate.
- The separation process was optimized by adjusting parameters such as electrokinetic injection and run buffer composition, achieving effective detection at specific wavelengths for each compound.
- A diode array UV detector enabled simultaneous detection of all three species, with nitrite and nitrate responding best at 214 nm and peroxynitrite at 302 nm for accurate identification.
Article Abstract
A method for the separation and direct detection of peroxynitrite (ONOO(-)) and two of its degradation products, nitrite (NO(2)(-)) and nitrate (NO(3)(-)), using capillary electrophoresis with ultraviolet detection is described. The separation parameters were optimized and included electrokinetic injection, a run buffer consisting of 25 mM K(2)HPO(4) 7.5 mM DTAB, pH 12, and a field strength of -323 V/cm. A diode array UV detector was employed in these studies as it allowed the determination of all three species simultaneously. Nitrate and nitrite provided the maximum response at 214 nm while peroxynitrite generated the best response at 302 nm. All three species could be detected at 214 nm, while simultaneous detection at 214 and 302 nm positively identified each peak.
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| http://dx.doi.org/10.1016/j.chroma.2005.05.027 | DOI Listing |
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