Purpose: The purpose of this study was to investigate the shear bond strength of 2 resin-based pit-and-fissure sealants--Clinpro and Fluroshield--to saliva-contaminated and noncontaminated enamel.
Methods: Forty buccal halves of permanent molar crowns were individually embedded in polyester resin and ground with wet silicone carbide papers to obtain flat enamel surfaces. The specimens were randomly assigned to 2 groups: (A) without contamination; and (B) contaminated with 0.01 ml of fresh human saliva. Each group was divided into 2 subgroups (N=10), according to the sealant applied: (1) Clinpro; and (2) Fluroshield. Shear bond strength was tested at a crosshead speed of 0.5 mm/min. Failure mode was assessed.
Results: Means (MPa) were: (1) A1=7.66 +/- 3.12; A2=12.39 +/- 4.34; (2) B1=5.05 +/- 1.44; B2=10.44 +/- 2.35. Data were submitted to analysis of variance and Scheffé's statistical test (P<.05). There was a statistically significant difference (P<.05) between both the sealants and the experimental conditions analyzed. Fluroshield provided higher bond strength and was different from Clinpro (P<.05) in the absence of contamination. Within the saliva-contaminated group, however, no statistically significant difference (P>.05) was observed between the tested materials.
Conclusions: It may be concluded that, under dry conditions, the filled pit-and-fissure sealant (Fluroshield) yielded better bonding performance. Salivary contamination undermined the adhesion of both materials to enamel and resulted in lower bond strengths.
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Sci Rep
January 2025
Department of Conservative Dentistry and Endodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, 600077, India.
Polydopamine (PD), inspired by the wet adhesion mechanism of mussel foot proteins, has emerged as a promising adhesive material with wide-ranging applications. This study aimed to compare the adhesive properties of PD and Glass Ionomer Cement (GIC) on enamel and dentin substrates, evaluating PD's potential as an alternative adhesive in dental practice. A total of 120 human premolars were prepared, with 80 teeth allocated for Scanning Electron Microscopy (SEM) analysis and 40 teeth reserved for shear bond strength testing.
View Article and Find Full Text PDFACS Nano
January 2025
Louvain Institute of Biomolecular Science and Technology, UCLouvain, Croix du Sud, 4-5, bte L7.07.07, B-1348 Louvain-la-Neuve, Belgium.
The iron-regulated surface determinant protein B (IsdB) has recently been shown to bind to toll-like receptor 4 (TLR4), thereby inducing a strong inflammatory response in innate immune cells. Currently, two unsolved questions are (i) What is the molecular mechanism of the IsdB-TLR4 interaction? and (ii) Does it also play a role in nonimmune systems? Here, we use single-molecule experiments to demonstrate that IsdB binds TLR4 with both weak and extremely strong forces and that the mechanostability of the molecular complex is dramatically increased by physical stress, sustaining forces up to 2000 pN, at a loading rate of 10 pN/s. We also show that TLR4 binding by IsdB mediates time-dependent bacterial adhesion to endothelial cells, pointing to the role of this bond in cell invasion.
View Article and Find Full Text PDFBMC Oral Health
January 2025
Conservative Dentistry Department, Faculty of Oral and Dental Medicine, Future University, Cairo, Egypt.
Background: This study aimed to assess the influence of different pretreatment protocols and antioxidants application on the shear bond strength (SBS) of universal adhesive to sound (SoD) and caries-induced dentin (CID).
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J Dent
January 2025
DDS, MS, PhD, Department of Dentistry, Federal University of Rio Grande do Norte, Natal, Brazil. Electronic address:
Objective: To evaluate the influence of different cleaning methods, surface treatments, and aging on the repair bond strength to a CAD/CAM glass-ceramic.
Materials And Methods: Forty-eight lithium disilicate CAD/CAM ceramic blocks were fabricated, sintered, and embedded in acrylic resin. After contamination with human saliva, they were divided according to the factors "Cleaning method" (Control-water/air spray, Air-particle abrasion with AlO, Ivoclean cleaning paste), "Surface treatment" (5% Hydrofluoric acid-HF + Silane, Monobond Etch & Prime-MEP), and "Aging" (thermocycling, no thermocycling).
J Thromb Haemost
January 2025
Department of Pathology and Laboratory Medicine; Institute of Reproductive Medicine and Developmental Sciences, The University of Kansas Medical Center, Kansas City, KS 66160. Electronic address:
Background: A loss-of-functional mutation (W1183R) in human complement factor H (CFH) is associated with complement-associated hemolytic uremic syndrome; mice carrying a similar mutation (W1206R) in CFH also develop thrombotic microangiopathy but its plasma von Willebrand factor (VWF) multimer sizes were dramatically reduced. The mechanism underlying such a dramatic change in plasma VWF multimer distribution in these mice is not fully understood.
Objective And Methods: To determine the VWF and CFH interaction and how CFH proteins affect VWF multimer distribution, we employed recombinant protein expression, purification, and various biochemical and biophysical tools.
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