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Development and Application of a Fully Automated Chemiluminescence Enzyme Immunoassay for the Detection of Antibodies Against Porcine Circovirus 3 Cap.
Viruses
December 2024
State Key Laboratory of Swine and Poultry Breeding Industry, National Engineering Center for Swine Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Porcine circovirus 3 (PCV3) is a small non-enveloped circovirus associated with porcine dermatitis and nephropathy syndrome (PDNS). It has occurred worldwide and poses a serious threat to the pig industry. However, there is no commercially available vaccine.
View Article and Find Full Text PDFImmunogenicity and Protective Efficacy of Baculovirus-Expressed SARS-CoV-2 Envelope Protein in Mice as a Universal Vaccine Candidate.
Vaccines (Basel)
August 2024
Department of Virology, Faculty of Veterinary Medicine, Ankara University, Ankara 06070, Türkiye.
The envelope (env) protein of SARS-CoV-2, a pivotal component of the viral architecture, plays a multifaceted role in viral assembly, replication, pathogenesis, and ion channel activity. These features make it a significant target for understanding virus-host interactions and developing vaccines to combat COVID-19. Recent structural studies provide valuable insights into the conformational dynamics and membrane topology of the SARS-CoV-2 env protein, shedding light on its functional mechanisms.
View Article and Find Full Text PDFA streamlined, automated workflow to screen and triage large numbers of baculoviruses for protein expression.
Protein Expr Purif
March 2024
Protein & Structural Chemistry, Discovery Chemistry, Merck & Co., Inc., 770 Sumneytown Pike, West Point, PA 19486, USA. Electronic address:
The baculovirus expression system is a powerful and widely used method to generate large quantities of recombinant protein. However, challenges exist in workflows utilizing either liquid baculovirus stocks or the Titerless Infected-Cells Preservation and Scale-Up (TIPS) method, including the time and effort to generate baculoviruses, screen for protein expression and store large numbers of baculovirus stocks. To mitigate these challenges, we have developed a streamlined, hybrid workflow which utilizes high titer liquid virus stocks for rapid plate-based protein expression screening, followed by a TIPS-based scale-up for larger protein production efforts.
View Article and Find Full Text PDFEnd-to-End Semi-automated Mid-scale Protein Screening Platform for Drug Discovery Research.
Curr Protoc
September 2023
Biomolecular Research, Genentech, South San Francisco, California.
The drug discovery landscape is ever-evolving and constantly demands revolutionary technology advancements in protein expression and production laboratories. We have built a higher-throughput mid-scale semi-automated protein expression and screening platform to accelerate drug discovery research. The workflow described here enables comprehensive expression and purification screening assessment of challenging or difficult-to-express recombinant proteins in a fast and efficient manner by delivering small but sufficient amounts of high-quality proteins.
View Article and Find Full Text PDFLive-cell microscopy or fluorescence anisotropy with budded baculoviruses-which way to go with measuring ligand binding to M muscarinic receptors?
Open Biol
June 2022
Institute of Chemistry, University of Tartu, Ravila 14a, 50411 Tartu, Estonia.
Article Synopsis
- The M muscarinic acetylcholine receptor is a special type of protein in our body that scientists study because it relates to problems like drug addiction and mental illnesses.
- Researchers are testing two special fluorescent compounds that glow under certain lights to see how well they stick to this receptor.
- They created new methods using living cells and computer programs to easily analyze how well different substances can bind to the receptor, helping in the search for new medicines.
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