Objective: To establish a method to examine the contents of diosgenin of Smilax china L. in different fields ,and to evaluate the influence of the growing environment on its quality.
Methods: Kromasie C18 column was used. The mobile phase was CH3OH-H2O (94:6), flow rate: 1.0 ml/min,the detective wavelength was 210 nm.
Results: Under the chromatographic condition the diosgenin can be completely separated,in the range of 5 microg-15 microg diosgenin had fine linear responses The mean recovery was 98.47%, and the RSD was 1.01%.
Conclusions: The method was sensitive, simple and accurate. There were some correlations on the quality of Smilax china L. and growing environment.
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