AI Article Synopsis
- Researchers created two cDNA libraries from rice lines that have similar genetics and resistance to blast disease, focusing on their different responses to neck blast.
- They identified 90 unique cDNA clones, with 74 having known functions related to pathogen response and signaling based on database comparisons.
- RT-PCR confirmed expression differences in 17 of 26 selected cDNA clones, with this study being the first to examine neck blast resistance at the mRNA level, paving the way for deeper genetic research.
Article Abstract
Two cDNA libraries enriched for transcripts differentially expressed in plants of two rice lines with similar genetic backgrounds and same leaf blast resistance but different responses to neck blast using suppression subtractive hybridization (SSH). After differential screening and sequence analysis of the selected clones, 90 unique cDNA clones were found, of which 74 clones were with known functions according to the putative functions of their homologous genes in the database. They may be involved in pathogen response, signal transduction, transcription, etc. Expression differences of 17 out of the 26 selected cDNA clones in resistant and susceptible lines were confirmed by RT-PCR. Expression profilings of the 26 cDNA clones at the early stages after inoculation were also revealed by RT-PCR. This is the first report on the rice neck blast resistance at mRNA level and will facilitate the further study of genetic mechanism of neck blast resistance.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/S0379-4172(06)60048-6 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Identification of blast resistant finger millet (Eleusine coracana L.) genotype through phenotypic screening and molecular profiling.
Sci Rep
December 2024
Bioinformatics Laboratory, Research & Developmental Cell, Parul University, Vadodara, 391760, Gujarat, India.
Finger millet blast caused by Pyricularia grisea hinders crop's growth and is a serious threat to economic yield. It can lead to massive yield losses i.e.
View Article and Find Full Text PDFFirst Report of Cereal Cyst Nematode, , in wheat in Shaanxi, China.
Plant Dis
December 2024
Northwest A&F University, College of Plant Protection and State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling, Shaanxi, China;
Cereal cyst nematodes spp., are important pathogens of wheat (Toumi et al. 2018).
View Article and Find Full Text PDFA rare case of spinal myeloid sarcoma.
Surg Neurol Int
November 2024
Limburg Clinical Research Centre (LCRC), University of Hasselt, Hasselt, Belgium.
Background: Myeloid sarcoma (MS), a rare extramedullary tumor composed of myeloid blast cells, is classified by the World Health Organization as a subtype of acute myeloid leukemia (AML). Isolated, primary, or nonleukemic MS has an incidence of 2/1,000,000 adults and constitutes only 0.7% of all AML cases.
View Article and Find Full Text PDFDeep juvenile xanthogranuloma invading the left tensor fasciae latae muscle: a case report and a literature review.
J Clin Exp Hematop
December 2024
Division of Pediatrics and Perinatology, Department of Multidisciplinary Internal Medicine, School of Medicine, Tottori University Faculty of Medicine, Yonago, Japan.
Article Synopsis
- Juvenile xanthogranuloma (JXG) is a rare benign condition primarily affecting neonates and young children, often presenting as skin lesions, but intramuscular JXG is much less common, accounting for only 0.6% of cases.
- A case involving a 5-month-old girl showed a slow-growing lump in her left thigh, which was diagnosed as deep JXG after imaging, biopsy, and surgical resection.
- Histological findings revealed characteristics typical of JXG, and despite initial concerns about tumor margins, the patient has shown no signs of recurrence over 48 months post-surgery, highlighting the importance of proper diagnosis and monitoring.
Navigating diagnostic dilemmas: a rare presentation of extramedullary T-lymphoblastic leukemia/lymphoma with chronic myeloid leukemia.
J Hematop
December 2024
Department of Pathology, Microbiology, and Immunology University of Nebraska Medical Center, Omaha, NE, USA.
Chronic myeloid leukemia (CML) typically presents in the chronic phase. The blast crisis phase in CML predominantly comprises the myeloid phenotype, while B-cell lymphoblastic crisis is common among the lymphoid lineages. Presentation as a T-lymphoblastic crisis is exceptionally rare.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!