The ontogeny of high affinity GABAA and central benzodiazepine receptors in the mouse cerebellum was investigated by measuring [3H]muscimol and [3H]flunitrazepam binding to membrane preparations during postnatal development. In the P2 fraction, [3H]muscimol binding was much more abundant than [3H]flunitrazepam binding at all ages. [3H]muscimol Bmax exhibited a peak around postnatal day 25 while [3H]flunitrazepam binding did not follow a parallel course. These results can be explained by the preferential presence in cerebellum of certain variants of the different subunits of the GABAA receptor complex and with different topographical distributions of the different receptor subtypes. Development dependent changes of organelle distribution during subcellular fractionation also contributed to the described developmental pattern.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1097/00001756-199105000-00012 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Anxiety-like Behavior and GABAAR/BDZ Binding Site Response to Progesterone Withdrawal in a Stress-Vulnerable Strain, the Wistar Kyoto Rats.
Int J Mol Sci
June 2022
Departamento de Farmacobiología, Centro de Investigación y de Estudios Avanzados IPN (Cinvestav-IPN), Ciudad de México 07360, Mexico.
Stress susceptibility could play a role in developing premenstrual anxiety due to abnormalities in the hypothalamus-pituitary-adrenal (HPA) axis and impairments in the GABAA receptors' benzodiazepine (BDZ) site. Hence, we studied the stress-vulnerable Wistar Kyoto rat strain (WKY) to evaluate progesterone withdrawal (PW) effects on anxiety, HPA axis response, and to explore indicators of GABAA functionality in the BDZ site. For five days, ovariectomized WKY rats were administered 2.
View Article and Find Full Text PDFChronic neuroleptic treatment combined with a high fat diet elevated [3H] flunitrazepam binding in the cerebellum.
Prog Neuropsychopharmacol Biol Psychiatry
January 2022
Behavioral Neuropharmacology and Neuroimaging Laboratory on Addictions (BNNLA), Clinical Research Institute on Addictions, Department of Pharmacology and Toxicology, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY, USA; Department of Psychology, University at Buffalo, Buffalo, NY, USA. Electronic address:
Clinical and preclinical studies have shown dysfunctions in genetic expression and neurotransmission of γ-Aminobutyric acid (GABA), GABA receptor subunits, and GABA-synthesizing enzymes GAD and GAD in schizophrenia. It is well documented that there is significant weight gain after chronic neuroleptic treatment in humans. While there are limited studies on the effects of diet on GABA signaling directly, a change in diet has been used clinically as an adjunct to treatment for schizophrenic relief.
View Article and Find Full Text PDFEffects of acute and chronic administration of neurosteroid dehydroepiandrosterone sulfate on neuronal excitability in mice.
Drug Des Devel Ther
October 2016
Croatian Institute for Brain Research, Department of Neuroscience, School of Medicine, University of Zagreb, Zagreb, Croatia.
Background: Neurosteroid dehydroepiandrosterone sulfate (DHEAS) has been associated with important brain functions, including neuronal survival, memory, and behavior, showing therapeutic potential in various neuropsychiatric and cognitive disorders. However, the antagonistic effects of DHEAS on γ-amino-butyric acidA receptors and its facilitatory action on glutamatergic neurotransmission might lead to enhanced brain excitability and seizures and thus limit DHEAS therapeutic applications. The aim of this study was to investigate possible age and sex differences in the neuronal excitability of the mice following acute and chronic DHEAS administration.
View Article and Find Full Text PDF[Brain benzodiazepine receptors in humans and rats with alcohol addiction].
Zh Nevrol Psikhiatr Im S S Korsakova
December 2014
Objective: Benzodiazepine receptors (BDR) in synaptosomal and mitochondrial membranes from different brain areas of alcohol abused patients (postmortem) and the brain cortex of male rats (Vistar line) with different preference to alcohol were studied.
Methods: Synaptosomal and mitochondrial receptors of membranes from different brain areas of patients with alcohol addiction and controls were explored using radioreceptor analysis with selective ligands [3H]flunitrazepam and [3H]PK-11195. BDR in the rat brain were studied using [3H]flunitrazepam and [3H]Ro5-4864.
The influence of acute and long-term piracetam administration on the dynamics of rapid (non-specific, anxiolytic) and slow (specific, nootropic) behavioral drug effects, as well as on their interrelation with NMDA- and BDZ-receptors was studied in inbred mice strains differing in cognitive and emotional status--C57BL/6 and BALB/c. The BALB/c strain contained 17% less [3H]-flunitrazepam binding sites in frontal cortex and 22% less [3H]-MK801 binding sites in hippocampus as compared to those in C57BL/6 mice. Based on these data, BALB/c strain was used as a model of psychopathology, combining increased anxiety and cognitive deficit.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!